P1220-CTXM, a pKP048-related IncFIIK plasmid carrying blaCTX-M-14 and qnrB4. (September 2017)

• Record Type: Journal Article
• Authors: Zhang, Defu; Yin, Zhe; Zhao, Yuzong; Feng, Jiao; Jiang, Xiaoyuan; Zhan, Zhe; Wu, Weili; Chen, Weijun; Wang, Jinglin; Li, Jianrong; Zhou, Dongsheng
• Is Part Of: Future microbiology. Volume 12:Number 12(2017)
• Page Start: 1035
• Page End: 1043
• Publication Date: 2017-09
• Subjects: CTX-M-14 -- Klebsiella pneumoniae -- multidrug resistance -- p1220-CTXM -- plasmid; Microbiology -- Research -- Periodicals; 616.9041
• DOI: 10.2217/fmb-2017-0026
• Languages: English
• ISSNs: 1746-0913

Abstract

Aim: This study aimed to characterize plasmid-mediated antimicrobial resistance in clinical Klebsiella pneumoniae 1220 carrying bla CTX-M-14 and qnrB4 .Materials & methods: Plasmid p1220-CTXM was transformed from the 1220 isolate into Escherichia coli through conjugal transfer and then fully sequenced. Antimicrobial susceptibility was determined by VITEK.Results: p1220-CTXM was an IncFIIK plasmid genetically closely related to pKP048 and carried resistance markers including bla CTX-M-14, bla DHA-1, qnrB4, sul1 and qacEΔ1, all of which were harbored in a 35.7-kb multidrug-resistant region. bla CTX-M-14 was located in a truncated IS Ecp1 - bla CTX-M-14 - orf477 transposition unit, and qnrB4 and bla DHA-1 were in a truncated qnrB4 - bla DHA-1 region.Conclusion: This study provided the insight into the co-occurrence of bla CTX-M-14 and qnrB4 and the evolution of pKP048-related IncFIIK plasmids.