Emergence of two distinct subpopulations from Klebsiella pneumoniae grown in the stimulated microgravity environment. (September 2017)

• Record Type: Journal Article
• Authors: Wang, Haili; Li, Wenliang; Gu, Lixiao; Gao, Xiaofang; Ni, Bin; Deng, Haiteng; Yang, Ruifu; Han, Yanping
• Is Part Of: Future microbiology. Volume 12:Number 11(2017)
• Page Start: 939
• Page End: 951
• Publication Date: 2017-09
• Subjects: biofilm formation -- cellulose -- crystal violet staining -- HARVs -- high aspect ratio rotating wall vessels -- Klebsiella pneumoniae -- phenotypic heterogeneity -- quantitative proteomics -- SMG -- stimulated microgravity -- type 3 fimbriae -- yeast cell agglutination; Microbiology -- Research -- Periodicals; 616.9041
• DOI: 10.2217/fmb-2017-0032
• Languages: English
• ISSNs: 1746-0913

Abstract

Aim: To isolate and characterize the two phenotypically distinct subpopulations from Klebsiella pneumoniae clonal cultures grown in the simulate microgravity environment.Materials & methods: Here clonal culture of K. pneumoniae strain ATCC BAA-1705 was grown within a vertically rotating wall vessel bioreactor. Microscopic, colony staining, biofilm assays and quantitative proteomics were used to define the features of subpopulations.Results: Two subpopulations were isolated based on colony appearance and bacterial morphology and indicated the different capability of biofilm formation and antibiotics resistance.Conclusion: These findings would raise a possibility of understanding the adaptive roles of bacterial subpopulations formed under certain conditions from the viewpoint of population variation.