High transfection efficiency of neural stem cells with magnetofection. (March 2011)

• Record Type: Journal Article
• Authors: Sapet, Cédric; Laurent, Nicolas; de Chevigny, Antoine; Gourrierec, Loïc Le; Bertosio, Elodie; Zelphati, Olivier; Béclin, Christophe
• Is Part Of: Biotechniques. Volume 50:Number 3(2011)
• Page Start: 187
• Page End: 189
• Publication Date: 2011-03
• Subjects: Magnetofection -- transfection -- neural stem cells -- NSC -- NeuroMag -- neuronal differentiation; Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/000113628
• Languages: English
• ISSNs: 0736-6205

Abstract

Primary neural stem cells (NSCs) can be cultivated and differentiated in vitro but are difficult to transfect using conventional methods. We describe a simple and rapid magnetofection-based method suitable for the lab bench as well as for high-throughput projects. Our method yields high transfection efficiency and can be used for deciphering the genetic control of neural cell differentiation.