Identification of plant polysaccharides by MALDI-TOF MS fingerprinting after periodate oxidation and thermal hydrolysis. (15th September 2022)

• Record Type: Journal Article
• Authors: Pandeirada, Carolina O.; Hageman, Jos A.; Janssen, Hans-Gerd; Westphal, Yvonne; Schols, Henk A.
• Is Part Of: Carbohydrate polymers. Volume 292(2022)
• Publication Date: 2022-09-15
• Subjects: ABN sugar beet arabinan -- AC autoclave -- AC121-pOx-PS periodate-oxidized polysaccharide treated with AC at 121 °C -- AC134-pOx-PS periodate-oxidized polysaccharide treated with AC at 134 °C -- AX arabinoxylan -- BWX birch wood xylan -- Cel cellulose -- DHB 2, 5-dihydroxybenzoic acid -- DO degree of oxidation -- DORel relative DO -- DOTheo theoretical maximum DO -- DP degree of polymerization -- ESI electron spray ionization -- GC gas chromatography -- GGM guar GM -- GM galactomannan -- HCA hierarchical cluster analysis -- HCl hydrochloric acid -- HG homogalacturonan (lemon pectin) -- Hn hexose oligomer -- HPAEC-PAD high-performance anion-exchange chromatography with pulsed amperometric detection -- HPLC high-performance LC -- HPSEC-RI high performance size exclusion chromatography with refractive index detection -- IO4− periodate -- LBGM locust bean GM -- LC liquid chromatography -- MALDI-TOF MS Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry -- MLG barley mixed-linked β-glucan -- MS mass spectrometry -- Mw molecular weight -- m/z mass-to-charge ratio -- ox-DPn oxidized oligosaccharide cluster region potentially with a DP n -- PC principal component -- PCA principal component analysis -- Pn pentose oligomer -- pOx-PS periodate-oxidized PS -- PS polysaccharide -- RG-I potato rhamnogalacturonan type I -- RT room temperature -- Rt retention time -- RAX rye AX -- TFA trifluoroacetic acid -- UA uronic acids -- uHexAnm methyl-esterified unsaturated GalA-oligomer with n GalA units and m methyl-esters -- UHPLC ultra-high-performance liquid chromatography -- WAX wheat AX -- WS wheat starch -- XG tamarind seed xyloglucan; Plant polysaccharides recognition -- Periodate oxidation -- Oxidized oligosaccharides -- MALDI-TOF MS; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2022.119685
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: An autoclave treatment at 121 °C on periodate-oxidized plant polysaccharides and mixes thereof was investigated for the release of oligosaccharides to obtain a generic polysaccharide depolymerization method for polysaccharides fingerprinting. Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) analysis of the oligosaccharides released showed that each polysaccharide had a unique oligosaccharides profile, even the same type of polysaccharide derived from different sources and/or having different fine structures ( e.g. class of (arabino)xylans, galactomannans, glucans, or pectic materials). Various polysaccharide classes present in a polysaccharide mix could be identified based on significantly different ( p < 0.05) marker m / z values present in the mass spectrum. Principal component analysis and hierarchical cluster analysis of the obtained MALDI-TOF MS data highlighted the structural heterogeneity of the polysaccharides studied, and clustered polysaccharide samples with resembling oligosaccharide profiles. Our approach represents a step further towards a generic and accessible identification of plant polysaccharides individually or in a mixture. Graphical abstract: Unlabelled Image Highlights: Thermal depolymerization of periodate-oxidized plant polysaccharides was studied. Structure-dependent oligosaccharides were obtained for various polysaccharides. MALDI-TOF MS was used as a fingerprinting technique for oxidized oligosaccharides. PCA and HCA of MALDI-TOF MS data distinguished polysaccharides.