Diversity and phylogenetic analysis of endosymbionts from Trioza erytreae (Del Guercio) and its parasitoids in Kenya. (26th July 2020)

• Record Type: Journal Article
• Authors: Rasowo, Brenda A.; Copeland, Robert S.; Khamis, Fathiya M.; Aidoo, Owusu F.; Ajene, Inusa J.; Mohamed, Samira A.; Sétamou, Mamoudou; Ekesi, Sunday; Borgemeister, Christian
• Is Part Of: Journal of applied entomology. Volume 145:Number 1/2(2021)
• Page Start: 104
• Page End: 116
• Publication Date: 2020-07-26
• Subjects: diversity -- endosymbionts -- parasitoids -- phylogeny -- Trioza erytreae; Entomology -- Periodicals; Insect pests -- Periodicals; 595.7
• DOI: 10.1111/jen.12807
• Languages: English
• ISSNs: 0931-2048

Abstract

Abstract: The African citrus triozid (ACT), Trioza erytreae (Del Guercio), is the primary vector of Candidatus Liberibacter africanus (CLaf), the causative agent of Africa citrus greening disease (ACGD). This study evaluates the diversity of ACT parasitoids and further characterizes endosymbionts associated with both T. erytreae and its parasitoids that could be used as biological control agents of T. erytreae and management of ACGD. Mitochondrial cytochrome oxidase I gene was used to reconstruct T. erytreae and its parasitoids phylogeny, while 16S rRNA gene was used for the bacterial phylogeny. One well‐supported clade of ACT was detected within the Triozidae phylogeny, while the parasitoid species clustered into four groups within eulophid and encyrtid phylogeny. The phylogenetic result of parasitoids was supported by morphological identification where five different parasitoid species could be identified, that is Tamarixia dryi, Psyllaephagus pulvinatus, Tetrastichus sp., Aphidencyrtus cassatus and Charipine species. Moreover, four eubacterial symbionts ( Wolbachia, Rickettsia, Arsenophonus and Candidatus Liberibacter sp.) were detected in T. erytreae and three symbionts ( Wolbachia, Rickettsia and Cardinuim ) in the parasitoid specimens. Maximum likelihood phylogenetic inferences clustered the identified eubacterial symbionts within α and γ proteobacteria subdivisions. Phylogenetic inferences of 16S rRNA gene sequences indicated that Wolbachia strains from ACT and the parasitoids did not form a single monophyletic clade; however, both clustered within Supergroup B. The impacts of these parasitoid species and endosymbionts on ACT are still unknown, but their occurrence and broad distribution indicate the possibility of future use for control of T. erytreae .