Ciliary melanin‐concentrating hormone receptor 1 (MCHR1) is widely distributed in the murine CNS in a sex‐independent manner. Issue 10 (12th June 2020)

• Record Type: Journal Article
• Authors: Diniz, Giovanne B.; Battagello, Daniella S.; Klein, Marianne O.; Bono, Bianca S. M.; Ferreira, Jozélia G. P.; Motta‐Teixeira, Livia C.; Duarte, Jessica C. G.; Presse, Françoise; Nahon, Jean‐Louis; Adamantidis, Antoine; Chee, Melissa J.; Sita, Luciane V.; Bittencourt, Jackson C.
• Is Part Of: Journal of neuroscience research. Volume 98:Issue 10(2020)
• Page Start: 2045
• Page End: 2071
• Publication Date: 2020-06-12
• Subjects: GPCR -- MCH -- neurochemical characterization -- olfactory integration -- RRID:AB_10000342 -- RRID:AB_10000347 -- RRID:AB_10680290 -- RRID:AB_1079363 -- RRID:AB_11218957 -- RRID:AB_2088494 -- RRID:AB_2143948 -- RRID:AB_2143957 -- RRID:AB_2296529 -- RRID:AB_2298772 -- RRID:AB_2313606 -- RRID:AB_2336615 -- RRID:AB_2336819 -- RRID:AB_2337258 -- RRID:AB_2340593 -- RRID:AB_2534082 -- RRID:AB_2534102 -- RRID:AB_2541682 -- RRID:AB_2552692 -- RRID:AB_2556542 -- RRID:AB_2556546 -- RRID:AB_2650437 -- RRID:AB_2650444 -- RRID:AB_2650446 -- RRID:AB_310305 -- RRID:AB_477010 -- RRID:AB_518526 -- RRID:AB_572219 -- RRID:AB_572248 -- RRID:AB_572268 -- RRID:AB_91683 -- RRID:SCR_014199 -- volume transmission; Neurobiology -- Periodicals; 612
• DOI: 10.1002/jnr.24651
• Languages: English
• ISSNs: 0360-4012

Abstract

Abstract: Melanin‐concentrating hormone (MCH) is a ubiquitous vertebrate neuropeptide predominantly synthesized by neurons of the diencephalon that can act through two G protein‐coupled receptors, called MCHR1 and MCHR2. The expression of Mchr1 has been investigated in both rats and mice, but its synthesis remains poorly described. After identifying an antibody that detects MCHR1 with high specificity, we employed immunohistochemistry to map the distribution of MCHR1 in the CNS of rats and mice. Multiple neurochemical markers were also employed to characterize some of the neuronal populations that synthesize MCHR1. Our results show that MCHR1 is abundantly found in a subcellular structure called the primary cilium, which has been associated, among other functions, with the detection of free neurochemical messengers present in the extracellular space. Ciliary MCHR1 was found in a wide range of areas, including the olfactory bulb, cortical mantle, striatum, hippocampal formation, amygdala, midline thalamic nuclei, periventricular hypothalamic nuclei, midbrain areas, and in the spinal cord. No differences were observed between male and female mice, and interspecies differences were found in the caudate‐putamen nucleus and the subgranular zone. Ciliary MCHR1 was found in close association with several neurochemical markers, including tyrosine hydroxylase, calretinin, kisspeptin, estrogen receptor, oxytocin, vasopressin, and corticotropin‐releasing factor. Given the role of neuronal primary cilia in sensing free neurochemical messengers in the extracellular fluid, the widespread distribution of ciliary MCHR1, and the diverse neurochemical populations who synthesize MCHR1, our data indicate that nonsynaptic communication plays a prominent role in the normal function of the MCH system. Abstract : Here we report the distribution of a receptor found in primary cilia, describing an unprecedent wide distribution in the nervous system, differences between rodent species, and a lack of differences between males and females. Our results suggest melanin‐concentrating hormone may be used to target cilia in a large number of areas of the nervous system.