Skull diploë is rich in aquaporin-4. Issue 1 (January 2020)

• Record Type: Journal Article
• Authors: Suzuki, Yuji; Kitaura, Hiroki; Nakamura, Yukimi; Kakita, Akiyoshi; Huber, Vincent J.; Capozzoli, Nicholas; Kwee, Ingrid L.; Nakada, Tsutomu
• Is Part Of: Heliyon. Volume 6:Issue 1(2020)
• Publication Date: 2020-01
• Subjects: Anatomy -- Pathology -- Medical imaging -- Nuclear medicine -- Radiology -- Tissue culture -- Bone -- Aquaporin 4 (AQP4) -- Skull -- Positron emission tomography(PET) -- N-(1, 3, 4-thiadiazol-2-yl) pyridine-3-[11C]-carboxamide ([11C]TGN-020) -- Immunohistology; Research -- Periodicals; Medical sciences -- Periodicals; Natural history -- Periodicals; Social sciences -- Periodicals; Earth sciences -- Periodicals; Physical sciences -- Periodicals; 507.2
• DOI: 10.1016/j.heliyon.2020.e03259
• Languages: English
• ISSNs: 2405-8440

Abstract

Abstract: Aquaporin-4 (AQP4) is a water conducting membrane integral protein channel which is widely expressed in the astrocyte system of the brain. During the development of the AQP4 positron emission tomography (PET) imaging agent [ 11 C]TGN-020 ( N -(1, 3, 4-thiadiazol-2-yl)pyridine-3-[ 11 C]-carboxamide), significant radioligand uptake was observed in the skull, where there was no known distribution of any aquaporin family proteins. Herein we confirmed via a newly developed method for bone-tissue immunohistology, a hitherto unrecognized distribution of AQP4, and not AQP1, in the skull. Other bony structures, by contrast, showed virtually no uptake of [ 11 C]TGN-020, and likewise, do not express either AQP4 or AQP1. Immunohistological analysis demonstrated that the AQP4 expression in the skull is restricted to the diploë. Consequently, we suspect AQP4 plays a pivotal role in the formation and maintenance of yellow marrow and the diploë. However, elucidating the exact nature of that role will require further studies. Abstract : Anatomy; Pathology; Medical imaging; Nuclear medicine; Radiology; Tissue culture; Bone; Aquaporin 4 (AQP4); Skull; Positron emission tomography(PET); N-(1, 3, 4-thiadiazol-2-yl) pyridine-3-[11C]-carboxamide ([11C]TGN-020); Immunohistology.