Front Cover: Bifunctional DNA Duplexes Permit Efficient Incorporation of pH Probes into Liposomes (ChemBioChem 15/2020). (13th July 2020)

• Record Type: Journal Article
• Authors: Dolder, Nicolas; von Ballmoos, Christoph
• Is Part Of: Chembiochem. Volume 21:Number 15(2020)
• Page Start: 2086
• Page End: 2086
• Publication Date: 2020-07-13
• Subjects: carboxyfluorescein -- DNA conjugates -- liposomes -- membrane proteins -- pH sensors; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.202000425
• Languages: English
• ISSNs: 1439-4227

Abstract

Abstract : Proton translocation across biological membranes is an essential mechanism for important cellular tasks such as ATP production or nutrient uptake. Proteoliposomes containing pH‐sensitive fluorescent dyes are an important tool to investigate these processes. The cover illustrates a lipid vesicle with an embedded proton translocating F1 FO ATP synthase. The pH‐sensitive dye is anchored to the membrane by bifunctional DNA duplexes to ensure efficient incorporation. The DNA backbone on the outside can be cut enzymatically to yield proteoliposomes with the pH sensor selectively on the inside. More information can be found in the full paper by C. von Ballmoos and N. Dolder. Cover art by N.D.