Surface Display of Complex Enzymes by in Situ SpyCatcher‐SpyTag Interaction. (21st April 2020)

• Record Type: Journal Article
• Authors: Gallus, Sabrina; Peschke, Theo; Paulsen, Malte; Burgahn, Teresa; Niemeyer, Christof M.; Rabe, Kersten S.
• Is Part Of: Chembiochem. Volume 21:Number 15(2020)
• Page Start: 2126
• Page End: 2131
• Publication Date: 2020-04-21
• Subjects: biocatalysis -- Lpp-OmpA -- membrane proteins -- self-assembly -- surface display; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.202000102
• Languages: English
• ISSNs: 1439-4227

Abstract

Abstract: The display of complex proteins on the surface of cells is of great importance for protein engineering and other fields of biotechnology. Herein, we describe a modular approach, in which the membrane anchor protein Lpp‐OmpA and a protein of interest (passenger) are expressed independently as genetically fused SpyCatcher and SpyTag units and assembled in situ by post‐translational coupling. Using fluorescent proteins, we first demonstrate that this strategy allows the construct to be installed on the surface of E. coli cells. The scope of our approach was then demonstrated by using three different functional enzymes, the stereoselective ketoreductase Gre2p, the homotetrameric glucose 1‐dehydrogenase GDH, and the bulky heme‐ and diflavin‐containing cytochrome P450 BM3 (BM3). In all cases, the SpyCatcher‐SpyTag method enabled the generation of functional whole‐cell biocatalysts, even for the bulky BM3, which could not be displayed by conventional fusion with Lpp‐OmpA. Furthermore, by using a GDH variant carrying an internal SpyTag, the system could be used to display an enzyme with unmodified N‐ and C‐termini. Abstract : Room for independent folding . A surface display method employs the SpyCatcher‐SpyTag system for post‐translational in situ coupling of independently expressed and properly folded passenger enzymes and membrane anchors. The efficient surface display of delicate and complex enzymes expands the toolbox for the fabrication of productive whole‐cell biocatalysts.