Multiplexed Detection of Analytes on Single Test Strips with Antibody‐Gated Indicator‐Releasing Mesoporous Nanoparticles. Issue 52 (1st October 2020)

• Record Type: Journal Article
• Authors: Climent, Estela; Biyikal, Mustafa; Gröninger, Delia; Weller, Michael G.; Martínez‐Máñez, Ramón; Rurack, Knut
• Is Part Of: Angewandte Chemie international edition. Volume 59:Issue 52(2020)
• Page Start: 23862
• Page End: 23869
• Publication Date: 2020-10-01
• Subjects: explosives -- hybrid materials -- multiplexing -- signal amplification -- test-strip analysis; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.202009000
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Rapid testing methods for the use directly at a point of need are expected to unfold their true potential especially when offering adequate capabilities for the simultaneous measurement of multiple analytes of interest. Considering the unique modularity, high sensitivity, and selectivity of antibody‐gated indicator delivery (gAID) systems, a multiplexed assay for three small‐molecule explosives (TATP, TNT, PETN) was thus developed, allowing to detect the analytes simultaneously with a single test strip at lower ppb concentrations in the liquid phase in <5 min using a fluorescence reader or a smartphone for readout. While the TNT and PETN systems were newly developed here, all the three systems also tolerated harsher matrices than buffered aqueous model solutions. Besides a single‐track strip, the outstanding modularity of the hybrid biosensor materials in combination with strip‐patterning technologies allowed us to obtain a multichannel strip in a straightforward manner, offering comparable analytical performance while allowing to be tailored even more to the user's need. Abstract : The three small‐molecule explosives TATP, TNT and PETN can be detected simultaneously with a single test strip at lower ppb concentrations in the liquid phase in <5 min through the employment of tailored immunochemically gated hybrid materials using mobile devices for readout.