Cover Picture: Engineered SAM Synthetases for Enzymatic Generation of AdoMet Analogs with Photocaging Groups and Reversible DNA Modification in Cascade Reactions (Angew. Chem. Int. Ed. 1/2021). Issue 1 (15th December 2020)

• Record Type: Journal Article
• Authors: Michailidou, Freideriki; Klöcker, Nils; Cornelissen, Nicolas V.; Singh, Rohit K.; Peters, Aileen; Ovcharenko, Anna; Kümmel, Daniel; Rentmeister, Andrea
• Is Part Of: Angewandte Chemie international edition. Volume 60:Issue 1(2021)
• Page Start: 1
• Page End: 1
• Publication Date: 2020-12-15
• Subjects: bioorthogonal -- MAT -- photocaging -- protein engineering -- SAM; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.202015604
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract : Sequence‐specific modification ("writing") and light‐triggered removal ("erasing") of DNA is reported for the first time in an enzymatic cascade. In their Research Article on page 480, Andrea Rentmeister and co‐workers engineered SAM synthetases suitable for converting methionine analogs with photo‐cleavable groups and show their compatibility with methyltransferases to provide DNA with a light‐sensitive "cage". Crystal structure analysis revealed how substitutions in the active site led to accommodation of the photo‐cleavable group.