Cover Feature: Responsive Fluorophore Aggregation Provides Spectral Contrast for Fluorescence Lifetime Imaging (ChemBioChem 15/2020). (16th July 2020)

• Record Type: Journal Article
• Authors: Schleyer, Kelton A.; Datko, Benjamin D.; Burnside, Brandon; Cui, Chao; Ma, Xiaowei; Grey, John K.; Cui, Lina
• Is Part Of: Chembiochem. Volume 21:Number 15(2020)
• Page Start: 2087
• Page End: 2087
• Publication Date: 2020-07-16
• Subjects: fluorescence lifetime -- fluorescein -- cyanobenzothiazole -- self-assembly; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.202000426
• Languages: English
• ISSNs: 1439-4227

Abstract

Abstract : Fluorophores are greatly influenced by their environment . In their full paper, L. Cui et al. show that triggering the aggregation of a self‐condensing scaffold labeled with a fluorophore induces changes in the emission spectrum, polarization, and lifetime. The aggregates exhibited a shorter lifetime detectable in cells by fluorescence lifetime imaging microscopy, thus suggesting that triggered aggregation strategies can be used in the probes for various biological events through lifetime imaging.