Microbial community modulates electrochemical performance and denitrification rate in a biocathodic autotrophic and heterotrophic denitrifying microbial fuel cell. (January 2019)

• Record Type: Journal Article
• Authors: Vijay, Ankisha; Chhabra, Meenu; Vincent, Tessy
• Is Part Of: Bioresource technology. Volume 272(2019)
• Page Start: 217
• Page End: 225
• Publication Date: 2019-01
• Subjects: Microbial fuel cell -- Autotrophic denitrification -- Heterotrophic denitrification -- Microbial community analysis -- Power output; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2018.10.030
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Cathodic autotrophic (AD) and heterotrophic (HD) denitrification were compared. Power density (4.45 W m −3 ) was high in AD MFC. Nitrate removal rate (2.06 kg N O 3 - -N m −3 d −1 ) was high in HD MFC. High microbial diversity and some common species were found in both conditions. RT-PCR studies confirmed narG, nirS, and nosZ presence in both conditions. Abstract: A comparison of autotrophic (AD) and heterotrophic (HD) cathodic denitrification in a Microbial Fuel Cell (MFC) was made in this study. Denitrifying microbial consortia were developed from cow manure and soil and acclimatized under AD and HD conditions. The AD MFC supported the power output of 4.45 W m −3 while removing nitrate nitrogen ( N O 3 - -N) at the rate of 0.118 kg N O 3 - -N m −3 d −1 . Significant power output (3.02 W m −3 ) and nitrate removal rate (2.06 kg N O 3 - -N m −3 d −1 ) were achieved in HD MFC. Further, 16S rDNA based community analysis revealed higher diversity in HDMFC. The genus Thauera and Pseudomonas were predominant in ADMFC while genus Klebsiella and Alkaliphilus were abundant in HDMFC. The abundance of the denitrifying genes namely narG, nirS, and nosZ were assessed with the help of quantitative PCR and presence of all the genes in both the conditions ensured the necessary molecular requirements for complete denitrification.