Comparison of sulphide and nitrate removal from synthetic wastewater by pure and mixed cultures of nitrate-reducing, sulphide-oxidizing bacteria. (January 2019)

• Record Type: Journal Article
• Authors: Watsuntorn, Wannapawn; Ruangchainikom, Chalermchai; Rene, Eldon R.; Lens, Piet N.L.; Chulalaksananukul, Warawut
• Is Part Of: Bioresource technology. Volume 272(2019)
• Page Start: 40
• Page End: 47
• Publication Date: 2019-01
• Subjects: NR-SOB -- Hydrogen sulphide oxidation -- Nitrate reduction -- Mixed culture -- Paracoccus spp.; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2018.09.125
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: New strains of nitrate-reducing, sulphide-oxidizing bacteria (NR-SOB) were isolated. Mixture of NR-SOB strains showed the highest N-NO3 − removal rate (5.5 mg N-NO3 − /L h) The NR-SOB strains were closely related to Paracoccus spp. (99% similarity) The pure and mixed strains of NR-SOB removed 100% gas-phase H2 S. Abstract: In this study, the activities of hydrogen sulphide (H2 S) oxidation and nitrate (N-NO3 − ) reduction by three pure and mixed strains of nitrate-reducing, sulphide oxidizing bacteria (NR-SOB) were determined. Batch experiments were performed at 35 °C and pH 7.0–8.0 with initial H2 S concentrations of 650–900 ppmv and N-NO3 − concentrations of ∼120 mg/L. The strains MAL 1HM19, TPN 1HM1 and TPN 3HM1 were capable of removing 100% gas-phase H2 S. The co-cultures showed better performance for H2 S and N-NO3 − removal. The mixed NR-SOB strains showed a higher H2 S oxidation rate (143 ± 18 ppmv /h), while the highest N-NO3 − removal rate (5.5 ± 0 and 5.1 ± 0.6 N-NO3 − mg/L·h) was obtained by a mixture of two NR-SOB strains. The 16S rDNA sequence analysis revealed that all strains belonged to the sub-class Alphaproteobacteria and are closely related to Paracoccus sp. (>99%).