Response of the aerobic denitrifying phosphorus accumulating bacteria Pseudomonas psychrophila HA-2 to low temperature and zinc oxide nanoparticles stress. (June 2022)

• Record Type: Journal Article
• Authors: Luo, Kongyan; Chen, Long; Du, Lei; Zhao, Yuanyi; Chen, Qian
• Is Part Of: Bioresource technology. Volume 354(2022)
• Publication Date: 2022-06
• Subjects: Denitrifying phosphate-accumulating organism -- Pseudomonas psychrophila -- ZnO nanoparticles -- Low temperature -- Resistance mechanism; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2022.127162
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Pseudomonas psychrophila HA-2 exhibited simultaneous N and P removal ability at 10 °C. ZnO NPs inhibited the N and P removal rates at 10℃ and facilitated P removal at 20 °C. Up-regulation of energy metabolism and ribosome made the psychrotolerance possible. ZnO NPs induced inhibition of purine and amino acid metabolisms at 10 °C. Antioxidative pathways were up-regulated to promote phosphorus accumulation at 20 °C. Abstract: Performance and molecular changes of an aerobic denitrifying phosphorus accumulating bacteria Pseudomonas psychrophila HA-2 have been investigated under different temperatures and ZnO nanoparticles (NPs) exposures. Strain HA-2 removed 95.7% of total nitrogen (TN) and 24.6% of phosphorus at 10 °C, which was attributed to the joint up-regulation of intracellular energy metabolism and ribosome. Moreover, with the increase of ZnO NPs from 0 to 100 mg/L, TN and phosphurs removal efficiencies decreased from 95.7% to 44.5% and 24.6% to 6.8% at 10 °C, respectively, whereas phosphorus removal rate increased from 10.5% to 24.5% at 20 °C. Further transcriptomics and proteomics revealed that significant down-regulation of purine and amino acid metabolisms was the main reason for the inhibitory effect at 10 °C, while the up-regulation of antioxidant pathways and functional genes expressions was responsible for the promoted phosphorus accumulation at 20 °C. This study provides a potential solution for improving biological nutrients removal processes in winter months.