Dual-stage biorefinery to convert spentwash hydrolysate into oleochemicals using Trichosporon cutaneum and Yarrowia lipolytica. (June 2022)

• Record Type: Journal Article
• Authors: V Sreeharsha, Rachapudi; Sai Tejaswini, G; Venkata Mohan, S
• Is Part Of: Bioresource technology. Volume 354(2022)
• Publication Date: 2022-06
• Subjects: Oleaginous yeast -- Biodiesel -- Sulphates -- Ammonium toxicity -- Lipids -- Fatty acid methyl esters; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2022.127146
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Distillery spentwash (SW) was used to produce lipids and VFAs by oleaginous yeasts. Trichosporon cutaneum produced 29.8% and 0.89 g/L lipids with acid hydrolysed SW. Dual-stage fermentation enhanced lipid productivity by 15% in Tc. Effect of sulphates and ammonium on lipid biosynthesis was elucidated. A biorefinery framework was elucidated to enable circular chemistry using yeast. Abstract: Yeast lipids from low-cost renewable feedstock are valuable resources for oleochemicals thus enabling circular chemistry. Current study focuses on lipid and volatile fatty acid (VFA) production through dual-stage fermentation of spentwash in a biorefinery framework with Trichosporon cutaneum ( Tc ) and Yarrowia lipolytica ( Yl ). During cell proliferation phase, Tc and Yl accumulated 2.9 and 2.5 g/L of dry biomass respectively in acid-hydrolysed spentwash (AHSW) and produced 16 and 5.5 g/L of total VFA respectively. Lipid yields (29.8%) and lipid titres (0.89 g/L) were higher in Tc /AHSW, when compared to Yl indicating the efficacy of Tc in spentwash bioremediation. Lipid accumulation was enhanced to 35% in Tc /AHSW, in presence of 0.05% NH4 Cl due to oxidative stress of ammonium ions. Analysis of fatty acid composition revealed the presence of higher oleic acid, which is ideal for biodiesel production. The results demonstrate a sustainable biorefinery model for bioremediation of spentwash and its value addition.