Bone-targeted ICG/Cyt c@ZZF-8 nanoparticles based on the zeolitic imidazolate framework-8: a new synergistic photodynamic and protein therapy for bone metastasis. (6th April 2022)

• Record Type: Journal Article
• Authors: Jiang, Zichao; Pan, Yixiao; Wang, Jiahao; Li, Jingyi; Yang, Haoze; Guo, Qi; Liang, Shuailong; Chen, Sijie; Hu, Yihe; Wang, Long
• Is Part Of: Biomaterials science. Volume 10:Number 9(2022)
• Page Start: 2345
• Page End: 2357
• Publication Date: 2022-04-06
• Subjects: Biomedical materials -- Periodicals; 610.28
• DOI: 10.1039/d2bm00185c
• Languages: English
• ISSNs: 2047-4830

Abstract

Abstract : Schematic diagram of (a) the synthesis of ICG/Cyt c@ZZF-8@PVP NPs and (b) the application of ICG/Cyc t@ZZF-8@PVP for bone-targeting synergistic therapy in mice. Abstract : Bone metastasis (BM) is a solid tumor confined to narrow bone marrow cavities with a relatively poor blood supply and hypoxic environment, making conventional anticancer treatments difficult. In our study, we fabricated nanoparticles (NPs) based on zeolitic imidazolate framework-8 (ZIF-8) loaded with indocyanine green (ICG, a photodynamic agent) and cytochrome c (Cyt c, an anticancer protein) with a surface modified by zoledronate (ZOL, a bone-targeting moiety) and a polyvinyl pyrrolidone (PVP) coating to increase their stability. The ICG/Cyt c@ZZF-8 NPs were expected to have synergistic antitumor therapy and bone protection efficiency. The in vitro and in vivo experiments showed the bone-targeted and pH-sensitive ability of ICG/Cyt c@ZZF-8 NPs, which could be engulfed by tumor cells and release the cargos. Upon 780 nm laser irradiation, ICG produces cytotoxic reactive oxygen species (ROS, 1 O2 ) that directly kill tumor cells, and Cyt c with catalase-like activity can induce programmed cell death and decompose H2 O2 to O2, thus enhancing the PDT efficiency. The ZOL can further inhibit bone resorption. The ICG/Cyt c@ZZF-8 NPs showed improved antitumor and bone protection efficiency in a mouse model of BM. This study demonstrated a potential mode for the synergetic therapy of orthopedic diseases.