Auto-cycling primer extension for amplified microRNA detection. Issue 40 (3rd May 2022)

• Record Type: Journal Article
• Authors: Wang, Huimin; Wang, Jiaoli; Liu, Ruiting; Liu, Zhenxiang; Wang, Kemin; Huang, Jin
• Is Part Of: Chemical communications. Volume 58:Issue 40(2022)
• Page Start: 6020
• Page End: 6023
• Publication Date: 2022-05-03
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d2cc01784a
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : We present a new isothermal nucleic acid amplification technique, termed auto-cycling primer extension (APE). It repeatedly extends short DNA primers to longer DNA hairpins, a DNA-based copy-and-release hairpin (CRH) and palindromic sequence design. Abstract : The current polymerase-based nucleic acid amplification techniques cannot maintain continuous polymerization reactions unless by changing the temperature or adding additional reagents ( e.g. the second enzyme or betaine), which undoubtedly increases the cost and operation steps. Herein, a new isothermal nucleic acid amplification technique, termed auto-cycling primer extension (APE), is presented. It repeatedly extends short DNA primers to longer DNA hairpins, by combining a DNA-based copy-and-release hairpin (CRH) and palindromic sequence design. The experimental results showed that we could realize the amplification detection of miRNA by a reasonable probe design.