Horizontal transfer of the multidrug resistance plasmid RP4 inhibits ammonia nitrogen removal dominated by ammonia-oxidizing bacteria. (15th June 2022)

• Record Type: Journal Article
• Authors: Zhao, Chen; Li, Jia; Li, Chenyu; Xue, Bin; Wang, Shang; Zhang, Xi; Yang, Xiaobo; Shen, Zhiqiang; Bo, Lin; Qiu, Zhigang; Wang, Jingfeng
• Is Part Of: Water research. Volume 217(2022)
• Publication Date: 2022-06-15
• Subjects: Antibiotic resistance gene -- Wastewater treatment plants -- Ammonia oxidation bacteria -- Ammonia removal; Water -- Pollution -- Research -- Periodicals; 363.7394
• DOI: 10.1016/j.watres.2022.118434
• Languages: English
• ISSNs: 0043-1354

Abstract

Highlights: AOB was a recipient of RP4 plasmid in SBR and pure AOB. Nitrite generation of pure AOB was decreased by RP4. Ammonia removal was significantly inhibited by RP4 whether in SBR and pure AOB. The expression of ammonia oxidation genes related to in AOB was suppressed by RP4. Abstract: Antibiotic resistance genes (ARGs) have become an important public health concern. Particularly, although several ARGs have been identified in wastewater treatment plants (WWTPs), very few studies have characterized their impacts on reactor performance. Therefore, our study sought to investigate the effect of a representative conjugative transfer plasmid (RP4) encoding multidrug resistance genes on ammonia oxidation. To achieve this, we established sequencing batch reactors (SBRs) and a conjugation model with E. coli donor strains carrying the RP4 plasmid and a typical ammonia-oxidating (AOB) bacterial strain ( Nitrosomonas europaea ATCC 25978) as a recipient to investigate the effect of conjugative transfer of plasmid RP4 on AOB. Our findings demonstrated that the RP4 plasmid carried by the donor strains could be transferred to AOB in the SBR and to Nitrosomonas europaea ATCC 25978. In SBR treated with donor strains carrying the RP4 plasmid, ammonia removal efficiency continuously decreased to 71%. Once the RP4 plasmid entered N. europaea ATCC 25978 in the conjugation model, ammonia removal was significantly inhibited and nitrite generation was decreased. Furthermore, the expression of several functional genes related to ammonia oxidation in AOB was suppressed following the transfer of the RP4 plasmid, including amoA, amoC, hao, nirK, and norB . In contrast, the cytL gene encoding cytochrome P460 was upregulated. These results demonstrated the ecological risk of ARGs in WWTPs, and therefore measures must be taken to avoid their transfer. Graphical abstract: Image, graphical abstract