A simple and sensitive electrochemiluminescence spectrum measurement platform and spectrum-resolved ratiometric sensor for miroRNA-141 determination. (1st August 2022)

• Record Type: Journal Article
• Authors: Kang, Qi; Huang, Yuan; Ma, Xuemei; Li, Mengmeng; Ma, Cheng; Shen, Dazhong
• Is Part Of: Electrochimica acta. Volume 422(2022)
• Publication Date: 2022-08-01
• Subjects: Electrochemiluminescence -- Spectrum measurement -- Analytical device -- Ratiometric sensor -- miroRNA determination; Electrochemistry -- Periodicals; Electrochemistry, Industrial -- Periodicals; 541.37
• DOI: 10.1016/j.electacta.2022.140544
• Languages: English
• ISSNs: 0013-4686

Abstract

Highlights: A new method for electrochemiluminescence (ECL) spectrum measurement was reported. Variable ECL intensity was converted to stable signal by an accumulation algorithm. The systematic error in ECL spectrum measurement via wavelength scan was corrected. A spectrum-resolved ratiometric ECL sensor was developed for miroRNA-141 detection. Abstract: The electrochemiluminescence (ECL) spectral information is useful in ECL mechanism investigations, ratiometric ECL sensors and multi-analyte determinations. To overcome the shortcomings of low sensitivity in charge coupled devices (CCD) ECL spectrometer and low time efficiency for spectrum discrimination by using single band-pass filter (BPF) in one potential scan, a simple and sensitive ECL spectrum measurement platform is developed by placing a portable BPF array turntable device (with eleven measurement channels and one indicator channel) between the working electrode and photomultiplier in a conventional ECL analyzer. The systematic error due to the time dependent ECL intensity, which is the main obstacle for ECL spectrum measurement in wavelength scan mode, is neutralized by using the accumulated intensity for each channel in a group of spectrum measurement cycles. In a spectrum-resolved ratiometric ECL sensor for miroRNA-141 determination, near-infrared ECL emitter of methionine-stabilized Au nanoclusters (Met-Au NCs) is used as the tag and blue ECL emitter of g-C3 N4 as the reference. The ratio of the total ECL intensity in near-infrared wavelength region (from Met-Au NCs) to that in blue wavelength region (from g-C3 N4 ) is in linear correlation with the logarithmic concentration of microRNA-141 from 1 fM to 10 pM, with the limit of detection of 0.22 fM. Graphical abstract: Schematic Illustration of spectrum-resolved ratiometric ECL sensor for miRNA-141 determination Image, graphical abstract