A new update of MALDI-TOF mass spectrometry in lipid research. (April 2022)

• Record Type: Journal Article
• Authors: Engel, Kathrin M.; Prabutzki, Patricia; Leopold, Jenny; Nimptsch, Ariane; Lemmnitzer, Katharina; Vos, D.R. Naomi; Hopf, Carsten; Schiller, Jürgen
• Is Part Of: Progress in lipid research. Volume 86(2022)
• Publication Date: 2022-04
• Subjects: MALDI-TOF MS -- Lipids -- Phospholipids -- Matrix -- Mass spectrometric imaging; 9-AA 9-Aminoacridine -- amu Atomar Mass Unit -- AgTFA Silver Trifluoroacetate -- APCI Atmospheric Pressure Chemical Ionization -- BN Boron Nitride -- CCS Cross Collisional Sections -- CE Cholesteryl Ester -- CHCA α-Cyano-4-Hydroxycinnamic Acid -- CI Chemical Ionization -- CID Collision-induced Dissociation -- CL Cardiolipin -- ClCCA 4-Chloro-α-Cyanocinnamic Acid -- DAG Diacylglycerol -- DAN 1, 5-diaminonaphthalene -- DBDA N1, N4-Dibenzylidenebenzene-1, 4-Diamine -- DCTB trans-2-[3-(4-Tertbutylphenyl)-2-Methyl-2-Propenylidene -- DESI Desorption Electrospray -- DGDG Digalactosyl-Diacylglycerol -- DHA 2, 5-Dihydroxyacetophenone -- DHB 2, 5-Dihydroxybenzoic Acid -- DIOS Desorption/Ionization on Porous Silicon -- DMAN 1, 8-Bis-(Dimethylamino)-Naphthalene -- DPH 1, 6-Diphenyl-1, 3, 5-Hexatriene -- EI Electron Ionization -- ESI Electrospray Ionization -- FA Fatty Acid -- FAB Fast Atom Bombardment -- FD Field Desorption -- FI Field Ionization -- FT Fourier Transformation -- GC Gas Chromatography -- GO Graphene Oxide -- GP Glycerophospholipid -- GSL Glycerosphingolipid -- HCD High Collision Dissociation -- HOCl Hypochlorous Acid -- HPLC High-Performance Liquid Chromatography -- HPTLC High-Performance Thin-layer Chromatography -- ICR Ion Cyclotron Resonance -- IHC Immunohistochemistry -- IMS Ion Mobility Spectrometry -- IR Infrared -- LC liquid chromatography -- LD Laser Desorption -- LDI Laser Desorption Ionization -- LMD Laser Microdissection -- LOD Level of Detection -- LPA Lysophosphatidic Acid -- LPC Lyso(monoacyl)-Phosphatidylcholine -- LPL Lyso(monoacyl)-Phospholipid -- MAG Monoacylglycerol -- MAI Matrix-Assisted Ionization -- MALDI Matrix-Assisted Laser Desorption and Ionization -- MGDG Monogalactosyl-Diacylglycerol -- MPLA Monophosphoryl Lipid A -- MS Mass Spectrometry -- MSI Mass Spectrometry Imaging -- MS/MS Tandem Mass Spectrometry -- MTBE Methyl-tButyl Ether -- MTPFPP meso-Tetrakis(Pentafluorophenyl)Porphyrin -- m/z Mass to Charge Ratio -- Nd:YAG Neodymium-Doped Yttrium Aluminum Garnet -- NMR Nuclear Magnetic Resonance -- oxPL Oxidized Phospholipid -- OzID Ozone-induced Dissociation -- PA Phosphatidic Acid -- PC Phosphatidylcholine -- PCA Principal Component Analysis -- PE Phosphatidylethanolamine -- PG Phosphatidylglycerol -- PI Phosphatidylinositol -- PIP2 Phosphatidylinositol-4, 5-bisphosphate -- PL Phospholipid -- PLA2 Phospholipase A2 -- PLC phospholipase C -- PNA para-Nitroaniline -- PIP Phosphatidylinositol-Phosphate -- POPC 1-Palmitoyl-2-Oleoyl-sn-Glycero-3-Phosphatidylcholine -- POPE 1-Palmitoyl-2-Oleoyl-sn-Glycero-3-Phosphatidylethanolamine -- PPI (Poly-)Phosphoinositides -- PS Phosphatidylserine -- PSD Post Source Decay -- QTOF Quadrupole Time-of-Flight -- Rf Retardation Factor -- ROI Region of Interest -- ROS Reactive Oxygen Species -- RP Reversed Phase -- S1P Sphingosine-1-Phosphate -- SALDI Surface-Assisted Laser Desorption/Ionization -- SIMS Secondary Ion MS -- SM Sphingomyelin -- sn Stereospecific Numbering -- S/N Signal to Noise Ratio -- SQDG Sulfoquinovosyl-diacylglycerol -- TAG Triacylglycerol -- TCNQ Tetracyanoquinomethane -- TFA Trifluoroacetic Acid -- Th Thomson -- THA 2, 4, 6-Trihydroxyacetophenone -- TLC Thin-Layer Chromatography -- TOF Time of Flight -- UV Ultraviolet.; Lipids -- Periodicals; Lipids -- Periodicals; Lipides -- Périodiques; Lipiden; 572.57
• DOI: 10.1016/j.plipres.2021.101145
• Languages: English
• ISSNs: 0163-7827

Abstract

Abstract: Matrix-assisted laser desorption and ionization (MALDI) mass spectrometry (MS) is an indispensable tool in modern lipid research since it is fast, sensitive, tolerates sample impurities and provides spectra without major analyte fragmentation. We will discuss some methodological aspects, the related ion-forming processes and the MALDI MS characteristics of the different lipid classes (with the focus on glycerophospholipids) and the progress, which was achieved during the last ten years. Particular attention will be given to quantitative aspects of MALDI MS since this is widely considered as the most serious drawback of the method. Although the detailed role of the matrix is not yet completely understood, it will be explicitly shown that the careful choice of the matrix is crucial (besides the careful evaluation of the positive and negative ion mass spectra) in order to be able to detect all lipid classes of interest. Two developments will be highlighted: spatially resolved Imaging MS is nowadays well established and the distribution of lipids in tissues merits increasing interest because lipids are readily detectable and represent ubiquitous compounds. It will also be shown that a combination of MALDI MS with thin-layer chromatography (TLC) enables a fast spatially resolved screening of an entire TLC plate which makes the method competitive with LC/MS.