Derivatizing Nile Red fluorophores to quantify the heterogeneous polarity upon protein aggregation in the cell. Issue 35 (13th April 2022)

• Record Type: Journal Article
• Authors: Sun, Rui; Wan, Wang; Jin, Wenhan; Bai, Yulong; Xia, Qiuxuan; Wang, Mengdie; Huang, Yanan; Zeng, Lianggang; Sun, Jialu; Peng, Congcong; Jing, Biao; Liu, Yu
• Is Part Of: Chemical communications. Volume 58:Issue 35(2022)
• Page Start: 5407
• Page End: 5410
• Publication Date: 2022-04-13
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d2cc00629d
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : A fluorescence sustained probe to reveal the micro-environment polarity change during the protein aggregation process. Abstract : Protein aggregation in the cell is often manifested by the formation of subcellular punctate structures. Herein, we modulated the solvatochromism and solubility of Nile Red fluorophore derivatives to quantitatively study the polarity inside pathogenic protein aggregates, revealing structure- and protein-dependent polarity heterogeneity.