Naturally occurring Anaplasma marginale infection in cattle: Molecular prevalence and associated risk factors, haemato-biochemical alterations, oxidant/antioxidant status and serum trace mineral levels. (June 2022)

• Record Type: Journal Article
• Authors: Das, Dhruba; Sarma, Kalyan; Eregowda, Chethan Gollahalli; Roychoudhury, Parimal; Rajesh, Justus Babykutty; Behera, Parthasarathi; Prasad, Hridayesh; Lalrinkima, Hniang; Aktar, Fatema; Bora, Nikitasha; Deka, Champak; Thakur, Neeraj; Tolenkhomba, Thingujam Chaa
• Is Part Of: Microbial pathogenesis. Volume 167(2022)
• Publication Date: 2022-06
• Subjects: Anaplasma marginale -- Cattle -- Prevalence -- Oxidative stress -- Haemolytic crisis; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2022.105575
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: The present investigation was undertaken to map the distribution of Anapalsma species infection in cattle from the Aizawl region of Mizoram, India, in relation to various risk factors, and to study the haemato-biochemical alterations, oxidant/antioxidant status and serum trace mineral levels in cattle with naturally occurring Anapalsma marginale infection. The study was carried out over 31 months from June 2019 to December 2021. A total of 401 cattle blood samples were collected and screened for the presence of Anaplasma spp. by microscopic examination and polymerase chain reaction (PCR). Non-infected clinically healthy cattle (n = 21) served as control. Blood samples were collected to study the haemogram and serum samples were used for the evaluation of biochemical parameters, oxidative stress indices and trace minerals. During the study period, an overall prevalence of 15.71% was recorded for A. marginale infection in cattle. The prevalence of A. marginale infection was highly associated with age, sex, breed and tick infestation status of animals, floor system and management of farms, and season. The mean values of total erythrocyte count (TEC), haemoglobin (Hb), packed cell volume (PCV), total platelet count, total protein, albumin, superoxide dismutase (SOD), glutathione (GSH), total antioxidant capacity (TAC), copper (Cu) and zinc (Zn) were significantly ( P < 0.05) lower, whereas the mean values of mean corpuscular volume (MCV), aspartate aminotransferase (AST), total bilirubin, indirect bilirubin, blood urea nitrogen (BUN), creatinine and lipid hydroperoxide (LPO) were significantly ( P < 0.05) higher in cattle infected with A. marginale . A negative correlation of TEC with LPO, and a positive correlation with SOD, GSH, TAC, Cu and Zn suggest a possible link between oxidative stress and the haemolytic crisis noticed in bovine anaplasmosis. Incorporation of antioxidants and organ protective drugs as an adjunct therapy may result in better prognosis. Highlights: This is the first report of molecular technique based study on prevalence of Anaplasma marginale infection in cattle of Mizoram, India. The presence of high amounts of LPO and low levels of SOD, GSH, TAC, Cu and Zn in affected cattle indicates oxidative stress and disrupted trace mineral homeostasis. A negative correlation of TEC with LPO, while a positive correlation with SOD, GSH, TAC, Cu and Zn suggest a possible association between oxidative stress and the haemolytic crisis.