A light-initiated chemical reporter strategy for spatiotemporal labeling of biomolecules. Issue 5 (8th April 2022)

• Record Type: Journal Article
• Authors: Wang, Feifei; Kong, Hao; Meng, Xiangfeng; Tian, Xiao; Wang, Changjiang; Xu, Lei; Zhang, Xiang; Wang, Lei; Xie, Ran
• Is Part Of: RSC chemical biology. Volume 3:Issue 5(2022)
• Page Start: 539
• Page End: 545
• Publication Date: 2022-04-08
• Subjects: 572
• DOI: 10.1039/d2cb00072e
• Languages: English
• ISSNs: 2633-0679

Abstract

Abstract : Combination bioorthogonal chemical reporter strategy and photo-reaction enables imaging of newly-synthesized glycans, lipids and proteins with spatiotemporal resolution. Abstract : The emergence of optochemical approaches has had a diverse impact over a broad range of biological research due to spatiotemporal regulation. Herein, we integrate this feature into the bioorthogonal chemical reporter strategy, which enables visible light-controlled spatiotemporal labeling of cell-surface glycans, lipids, and proteins. The metabolic precursors were first incorporated into live cells, and next the bioorthogonal reaction converted the azide/alkyne into a photo-active functional group, which allowed for subsequent photo-click reaction. We demonstrated this strategy by specifically labeling sialome, mucin-type O -glycome, phospholipids and newly-synthesized membrane proteins, respectively. The sequential photoirradiation-orthogonal reporter tagging (SPORT) should facilitate the probing of biomolecules in complex biological systems with high spatiotemporal precision.