Dietary supplementation with Weissella cibaria C-10 and Bacillus amyloliquefaciens T-5 enhance immunity against Aeromonas veronii infection in crucian carp (Carassiu auratus). (June 2022)

• Record Type: Journal Article
• Authors: Zhu, Xin-Kun; Yang, Bin-Tong; Hao, Zhi-Peng; Li, Hai-Zhou; Cong, Wei; Kang, Yuan-Huan
• Is Part Of: Microbial pathogenesis. Volume 167(2022)
• Publication Date: 2022-06
• Subjects: Crucian carp -- Bacillus amyloliquefaciens -- Weissella cibaria -- Probiotics -- Immune protection; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2022.105559
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: With the aim to discover novel lactic acid bacteria and Bacillus strains from fish as potential probiotics to replace antibiotics in aquaculture, the present study was conducted to isolate lactic acid bacteria and Bacillus from intestinal tract of healthy crucian carp ( Carassiu auratus ) and largemouth bass ( Micropterus salmoides ) and evaluate their resistance against Aeromonas veronii . Based on the evaluation of antibacterial activity and tolerance test, one strain of lactic acid bacteria ( Weissella cibaria C-10) and one strain of Bacillus ( Bacillus amyloliquefaciens T-5) with strong environmental stability were screened out. The safety evaluation showed that these two strains were non-toxic to crucian carp and were sensitive to most antibiotics. In vivo study, the crucian carps were fed a basal diet supplemented with W. cibaria C-10 (C-10), B. amyloliquefaciens T-5 (T-5) and W. cibaria C-10 + B. amyloliquefaciens T-5 (C-10+T-5), respectively, for 5 weeks. Then, various immune parameters were measured at 35 days of post-feeding. Results showed both probiotics could improve the activities of related immune enzymes, immune factors and non-specific immune antibodies in blood and organs (gill, gut, kidney, liver, and spleen) of crucian carp in varying degrees. Moreover, after 7 days of challenge experiment, the survival rates after challenged with A. veronii of W. cibaria C-10 (C-10), B. amyloliquefaciens T-5 (T-5) and W. cibaria C-10 + B. amyloliquefaciens T-5 (C-10+T-5) supplemented groups to the crucian carps were 20%, 33% and 22%, respectively. Overall, W. cibaria C-10 and B. amyloliquefaciens T-5 could be considered to be developed into microecological preparations for the alternatives of antibiotics in aquaculture. Highlights: W. cibaria C-10 and B. amyloliquefaciens T-5 with strong stability were screened out from intestinal tract of crucian carp. W. cibaria C-10 and B. amyloliquefaciens T-5 can improve the related immune indicators of crucian carp. W. cibaria C-10 and B. amyloliquefaciens T-5 enhanced protection against A. veronii TH0426 infection.