A thermo-sensitive and injectable hydrogel derived from a decellularized amniotic membrane to prevent intrauterine adhesion by accelerating endometrium regeneration. (1st April 2022)

• Record Type: Journal Article
• Authors: Li, Xiaoyu; Li, Peilin; Wang, Can; Shang, Ting; Han, Haotian; Tong, Yongjuan; Kang, Yubin; Fang, Jianjun; Cui, Lei
• Is Part Of: Biomaterials science. Volume 10:Number 9(2022)
• Page Start: 2275
• Page End: 2286
• Publication Date: 2022-04-01
• Subjects: Biomedical materials -- Periodicals; 610.28
• DOI: 10.1039/d1bm01791h
• Languages: English
• ISSNs: 2047-4830

Abstract

Abstract : An injectable and thermo-sensitive hydrogel was derived from AM, which can be injected and solidified in vivo . The dAM-gel offers an option for preventing IUA by promotion of the re-epithelialization of the damaged endometrium. Abstract : Objective To investigate the effect of the injectable hydrogel generated from a decellularized amniotic membrane (dAM)-gel on preventing the development of an intrauterine adhesion (IUA) on a rat model. Methods The dAM-gel was developed from an amniotic membrane (AM) by a process of decellularization, lyophilization, and enzyme digestion. Histological analysis, residual component determination, electronic microscopy and turbidimetric gelation kinetics analysis were performed to characterize the dAM-gel. The proliferation and migration of endometrial cells on the dAM-gel coated surface was examined. IUA was surgically created in rats and received dAM-gel injection immediately after wound creation. Gene profiles of epithelial cells cultured on the dAM-gel coated surface were evaluated by RNA-sequencing. Results The collagen content was retained in the dAM-gel, while the GAG content decreased significantly compared with fresh AM (fAM). Gelation of the gel was temperature-sensitive and showed a matrix concentration-dependent manner. Transplantation of the dAM-gel significantly reduced fibrosis of IUA with a recovered uterine cavity, regenerated endometrium and increased microvascular density, along with elevated pregnancy rate compared with endometrium damage groups. Migration of epithelial cells was greatly promoted by the dAM-gel in a surgically created uterine wound model. By comparing the RNA-sequence data of epithelial cells that were cultured on dAM-gel coated and non-coated surfaces, respectively, distinct gene profiles relative to the cellular migration, adhesion and angiogenesis and involved signaling pathway were identified. Conclusions The injectable dAM-gel developed from AM offers a promising option for preventing endometrial fibrosis by promotion of the re-epithelialization of the damaged endometrium.