DAT and PRX1 gene expression modulates vincristine production in Catharanthus roseus L. propagates using Cu, Fe and Zn nano structures. (July 2022)

• Record Type: Journal Article
• Authors: Perveen, Shaghufta; Safdar, Naila; Yasmin, Azra; Bibi, Yamin
• Is Part Of: Plant science. Volume 320(2022)
• Publication Date: 2022-07
• Subjects: DAT/PRX 1 genes expression -- Vincristine -- Nanoelicitation -- Catharanthus roseus -- Metallic nanostructures -- Terpenoid indole alkaloid (TIA); Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2022.111264
• Languages: English
• ISSNs: 0168-9452

Abstract

Abstract: Underlying mechanism of nanostructures upon monoterpene induction in Catharanthus roseus has not been explored yet. In the current study, Copper, Iron and Zinc nanoparticles were biosynthesized by Eriobotrya japonica seed extract and capped with reduced glutathione. Biosynthesized nanoparticles and their capped analogues were characterized by UV–visible spectrophotometer, FTIR, XRD and SEM. Selected concentration of nanostructures were used in plant tissue culture media which instigated the production of alkaloids, tannins and flavonoids without significantly affecting the growth index of propagated calli and shoots cultures of C. roseus . Accelerated vincristine production was noticed in propagated calli and shoots under copper and zinc nanostress (1645–1865 μg/ml respectively) with the least effect by iron nanostructure. Highest concentration of calcium was recorded in in vitro shoots under capped (3.42 mg/ml ± 7.16) and uncapped (4.41 mg/ml ± 20.44) Zn nanoparticles compared to control (2.82 mg/ml ± 13.41). Real time PCR depicts nano-zinc mediated increased expression of DAT and PRX1 genes of TIA pathway. Significant correlation among PRX1/DAT gene expression with vincristine production and calcium accumulation in the presence of nanostress validate by PCA. This study paved way the opportunities of metal biogenic nanomaterials as an ideal drug modulator in plant tissue culture studies. Graphical Abstract: ga1 Highlights: Copper, iron and zinc nanoparticles were synthesized by seed extract of Eriobutrya japonica and capped with glutathione. Biosynthesized nanostructures augmented vincristine in calli and shoot propagates of Catharanthus roseus . Zinc nanostress modulate PRX1 and DAT gene of terpenoid indole alkaloids pathway in shoots propagates. PCA corroborates positive relationship of PRX1 and DAT gene expression with vincristine production and calcium accumulation.