The effect of adding Bacillus amyloliquefaciens LSG2-8 in diets on the growth, immune function, antioxidant capacity, and disease resistance of Rhynchocypris lagowskii. Issue 125 (June 2022)

• Record Type: Journal Article
• Authors: Yu, Mengnan; Zhang, Yurou; Zhang, Dongming; Wang, Qiuju; Wang, Guiqin; Elsadek, Mahmound; Yao, Qi; Chen, Yuke; Guo, Zhixin
• Is Part Of: Fish & shellfish immunology. Issue 125(2022)
• Page Start: 258
• Page End: 265
• Publication Date: 2022-06
• Subjects: Rhynchocypris lagowskii -- Bacillus amyloliquefaciens -- antioxidant Capacity -- Aquaculture; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2022.05.008
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: The purpose of this study was to investigate the effect of Bacillus amyloliquefaciens LSG2-8 on the growth performance, immune function, antioxidant capacity, and disease resistance of Rhynchocypris lagowskii . Fish were fed with the feed containing five levels such as 0, 1.0 × 10 6, 1.0 × 10 7, 1.0 × 10 8, and 1.0 × 10 9 CFU/g of the B. amyloliquefaciens LSG2-8 for 56 days. After 56 days of feeding, twenty four fish were randomly selected to test various growth, immune and antioxidant parameters. Ten fish were challenged with Aeromonas hydrophila for 14 days; the mortality rate was recorded 14 days after infection. The results showed that B. amyloliquefaciens LSG2-8 could significantly increase the growth parameters of R. lagowskii's, such as final body weight, weight gain rate, specific growth rate, and feed efficiency (p < 0.05). Further examination revealed the activity of antioxidant enzymes, Nrf-2 mRNA, and Keap-1 mRNA gene expression in the intestine and liver, and the serum immune index of R. lagowskii in the 1.0 × 108 CFU/g were all significantly higher compared to the other groups. Furthermore, fish fed a diet supplemented with B. amyloliquefaciens LSG2-8 had a significantly lower (p < 0.05) post-challenge mortality rate than the control fish. In summary, the research results showed that B. amyloliquefaciens LSG2-8 could improve the growth performance, immune function, antioxidant capacity, and disease resistance of R. lagowskii and be used in aquaculture. Highlights: This study was the first to study the effect of fish-derived Bacillus amyloliquefaciens LSG2-8 on the growth, immune function, antioxidant capacity and disease resistance of Rhynchocypris lagowskii . The results show that adding a certain concentration of Bacillus amyloliquefaciens LSG2-8 to the diet of Rhynchocypris lagowskii can improve the growth, immune function, antioxidant capacity and disease resistance of Rhynchocypris lagowskii. This study provides basic data for aquatic microecological preparations.