Overexpression of long non-coding RNA ACTA2-AS1 inhibits the viability, proliferation, migration and invasion of colorectal cancer cells. (June 2022)

• Record Type: Journal Article
• Authors: Wu, Xiaoping; Pang, Qiujie; Zhi, Fuxiang; Mao, Xiaohong; Hu, Yingbin
• Is Part Of: Tissue & cell. Volume 76(2022)
• Publication Date: 2022-06
• Subjects: Colorectal Cancer -- Long non-coding RNA -- ACTA2 antisense RNA 1 -- Proliferation; Cytology -- Periodicals; 571.5
• DOI: 10.1016/j.tice.2022.101769
• Languages: English
• ISSNs: 0040-8166

Abstract

Abstract: The role of long non-coding RNA ACTA2 antisense RNA 1 (LncRNA ACTA2-AS1) in colorectal cancer (CRC) was awaited to be elucidated. Clinical specimen and data on ACTA2-AS1 expression in colon adenocarcinoma (COAD) were collected, followed by in situ hybridization. Transfected CRC cell viability, proliferation, migration, and invasion were determined with Cell Counting Kit-8, colony formation, Scratch, and Transwell assays, respectively. Relative expressions of ACTA2-AS1, PCNA, Bcl-2, MMP-2 and MMP-9 were quantified by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot. ACTA2-AS1 expression was downregulated in CRC. Overexpressed ACTA2-AS1 repressed the cell viability, proliferation, migration and invasion, increased cleaved caspase-3 level yet decreased PCNA, Bcl-2, MMP-2 and MMP-9 levels. ACTA2-AS1 silencing, however, did oppositely. Collectively, ACTA2-AS1 inhibits the viability, proliferation, migration and invasion of CRC cells to effectively suppress the progression of CRC. Highlights: ACTA2-AS1 is downregulated in CRC. Overexpressed ACTA2-AS1 represses CRC cell viability and proliferation. Overexpressed ACTA2-AS1 inhibits CRC cell migration and invasion. Silencing ACTA2-AS1 promotes CRC cell viability and proliferation Silencing ACTA2-AS1 enhances CRC cell migration and invasion.