Transcriptomic analysis reveals the gene expression profiles in the spleen of spotted knifejaw (Oplegnathus punctatus) infected by Vibrio harveyi. (August 2022)

• Record Type: Journal Article
• Authors: Wang, Jie; Chen, Zhangfan; Xu, Wenteng; Li, Yangzhen; Lu, Sheng; Wang, Lei; Song, Yu; Wang, Na; Gong, Zhihong; Yang, Qian; Chen, Songlin
• Is Part Of: Developmental and comparative immunology. Volume 133(2022)
• Publication Date: 2022-08
• Subjects: Oplegnathus punctatus -- Comparative transcriptome -- WGCNA -- Vibro harveyi -- Innate immunity -- Spleen; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2022.104432
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: As one of the most valuable maricultured species, spotted knifejaw ( Oplegnathus punctatus ) has high popularity in eastern Asia. In recent years, diseases caused by Vibrio harveyi have brought huge economic losses in spotted knifejaw industry. To better understand the molecular mechanisms of immune response about V. harveyi resistance in spotted knifejaw, a comparative transcriptome analysis was performed on spleen tissues at five different time points post-infection (0, 12, 24, 48 and 72 hpi). A total of 4279 differentially expressed genes (DEGs) were identified. KEGG pathways analysis showed that multiple immune-related pathways were significant regulated, including Toll-like receptor signaling pathway, ECM-receptor interaction pathway, cytokine-cytokine receptor interaction pathway and hematopoietic cell lineage pathway. Weighted gene co-expression network analysis showed that several immune-related pathways of the highest correlation with 12 hpi (cor = 0.89, P = 7e-06) were significantly enriched. In addition, 12 hpi was a turning point for 7 gene clusters out of 9 that were divided according to gene expression patterns. Therefore, we speculated that 12 hpi might be a very critical time point for spotted knifejaw against V. harveyi infection. Additionally, qRT-PCR was carried out to validate the expressions of 12 DEGs. This study provided the first systematical transcriptome analysis of spotted knifejaw against V. harveyi . The results could help us better understand the dynamic immune responses of spotted knifejaw against bacterial infection, and provide useful information for antibacterial defense in spotted knifejaw industry as well. Highlights: 4279 differentially expressed genes were identified in the spleen of spotted knifejaw infected with Vibrio harveyi. 12 hpi might be a critical time point for spotted knifejaw against V. harveyi infection. Multiple immune-related pathways were enriched in the infected spotted knifejaw. Several cytokines and chemokines were up-regulated by bacterial infection.