Anisodamine affects the pigmentation, mineral density, craniofacial area, and eye development in zebrafish embryos. Issue 6 (29th December 2021)

• Record Type: Journal Article
• Authors: Wang, Binjie; Chen, Tianyi; Wang, Anli; Fang, Jiakai; Wang, Jiye; Yao, Weixuan; Wu, Yuanzhao
• Is Part Of: Journal of applied toxicology. Volume 42:Issue 6(2022)
• Page Start: 1067
• Page End: 1077
• Publication Date: 2021-12-29
• Subjects: anisodamine -- craniofacial development -- pigmentation -- toxicology -- tropine alkaloidszebrafish; Toxicology -- Periodicals; Industrial toxicology -- Periodicals; Environmentally induced diseases -- Periodicals; Toxicology -- Periodicals; 615.9005
• DOI: 10.1002/jat.4278
• Languages: English
• ISSNs: 0260-437X

Abstract

Abstract: Anisodamine is one of the major components of the tropine alkaloid family and is widely used in the treatment of pain, motion sickness, pupil dilatation, and detoxification of organophosphorus poisoning. As a muscarinic receptor antagonist, the low toxicity and moderate drug effect of anisodamine often result in high doses for clinical use, making it important to fully investigate its toxicity. In this study, zebrafish embryos were exposed to 1.3‐, 2.6‐, and 5.2‐mM anisodamine for 7 days to study the toxic effects of drug exposure on pigmentation, mineral density, craniofacial area, and eye development. The results showed that exposure to anisodamine at 1.3 mM resulted in cranial malformations and abnormal pigmentation in zebrafish embryos; 2.6‐ and 5.2‐mM anisodamine resulted in significant eye development defects and reduced bone density in zebrafish embryos. The associated toxicities were correlated with functional development of neural crest cells through gene expression (col1a2, ddb1, dicer1, mab21l1, mab21l2, sox10, tyrp1b, and mitfa) in the dose of 5.2‐mM exposed group. In conclusion, this study provides new evidence of the developmental toxicity of high doses of anisodamine in aqueous solutions to organisms and provides a warning for the safe use of this drug. Abstract : We studied the early developmental toxicity of anisodamine in zebrafish. The results showed that anisodamine exposure resulted in toxicity such as increased pigmentation, decreased mineral density, smaller craniofacial area, and smaller eyes. Alterations in related genes were associated with the functional development of neural crest cells.