Genomic diversities of ctxB, tcpA and rstR alleles of Vibrio cholerae O139 strains isolated from Odisha, India. Issue 3 (19th October 2021)

• Record Type: Journal Article
• Authors: Behera, Dipti Ranjan; Nayak, Ashish Kumar; Nayak, Smruti Ranjan; Nayak, Dilena; Swain, Sipraswati; Maharana, Pradeep Kumar; Biswal, Bhagyalaxmi; Pany, Swatishree; Pati, Sanghamitra; Pal, Bibhuti Bhusan
• Is Part Of: Environmental microbiology reports. Volume 14:Issue 3(2022)
• Page Start: 376
• Page End: 384
• Publication Date: 2021-10-19
• Subjects: Microbial ecology -- Periodicals; Environmental Microbiology -- Periodicals; Electronic journals; 579.17
• DOI: 10.1111/1758-2229.13016
• Languages: English
• ISSNs: 1758-2229

Abstract

Summary: The genome of Vibrio cholerae O139 strains has undergone cryptic changes since its first emergence in 1992 in South India. This study aimed to determine the presence of genotypic changes marked in ctx B, tcp A and rst R genes located within the CTX prophages among the strains of V. cholerae O139 isolated from 1999 to 2017 in Odisha. Antibiotic susceptibility test was conducted on 59 V. cholerae O139 strains. A conventional PCR assay was done for ctx B gene typing followed by sequencing along with identification of rst R and tcp A gene. Pulsed‐field gel electrophoresis (PFGE) was carried out to reveal clonal variations among the V. cholerae O139 strains. Among V. cholerae O139 isolates more than 60% showed resistance to ampicillin, co‐trimoxazole, furazolidone, streptomycin, neomycin and nalidixic acid. The ctx B sequencing and rst R allele‐specific PCR assay revealed the presence of three genotypes 1, 3 and 4 with at least one copy of CTX Calc φ in addition to CTX ET and CTX Cl prophages in V. cholerae O139 isolates. PFGE analysis revealed 13 pulsotypes with two clades having 60% similarity among V. cholerae O139 strains. The circulating V. cholerae O139 strains in Odisha showed variation in genotypes with multiple clonal expansions over the years.