Design, Synthesis, and Characterization of a Novel Fluoroprobe for Live Human Islet Cell Imaging of Serotonin 5‐HT1A Receptor. (5th April 2022)

• Record Type: Journal Article
• Authors: Garvey, Robert W.; Lacivita, Enza; Niso, Mauro; Duszyńska, Beata; Harris, Paul E.; Leopoldo, Marcello
• Is Part Of: ChemMedChem. Volume 17:Number 10(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-04-05
• Subjects: serotonin -- serotonin 1A receptor -- fluorescent microscopy -- islets -- β-cells -- insulin -- vesicles -- diabetes; Pharmaceutical chemistry -- Periodicals; 615.19005
• DOI: 10.1002/cmdc.202100759
• Languages: English
• ISSNs: 1860-7179

Abstract

Abstract: Mounting evidence suggests that the serotonin system serves in signal transmission to regulate insulin secretion in pancreatic islets of Langerhans. Among the 5‐HT receptor subtype found in pancreatic islets, serotonin receptor 1A (5‐HT1A ) demonstrates a unique ability to inhibit β‐cell insulin secretion. We report the design, synthesis, and characterization of two novel fluorescent probes for the 5‐HT1A receptor. The compounds were prepared by conjugating the scaffold of the 5‐HT1A receptor agonist 8‐OH‐DPAT with two fluorophores suitable for live‐cell imaging. Compound 5a {5‐(dimethylamino)‐ N ‐[5‐[(8‐hydroxy‐1, 2, 3, 4‐tetrahydronaphthalen‐2‐yl)(propyl)amino]pentyl]naphtalen‐1‐sulfonammide} showed high affinity for the 5‐HT1A receptor ( K i =1.8 nM). Fluoroprobe 5a was able to label the 5‐HT1A receptor in pancreatic islet cell cultures in a selective manner, as the fluorescence emission was significantly attenuated by co‐administration of the 5‐HT1A receptor antagonist WAY‐100635. Thus, fluoroprobe 5a showed useful properties to further characterize this unique receptor's role. Abstract : We have developed a high‐affinity green‐emitting fluorescent probe for the serotonin 5‐HT1A receptor used for first‐time imaging of the 5‐HT1A receptor in live human islet cells. 5‐HT1 A receptors are colocalized with insulin in human β‐cells and with glucagon in human α‐cell vesicles.