Transcriptomic characterisation of a common skin lesion in farmed chinook salmon. Issue 124 (May 2022)

• Record Type: Journal Article
• Authors: Anderson, Kelli C.; Ghosh, Bikramjit; Chetty, Thaveshini; Walker, Seumas P.; Symonds, Jane E.; Nowak, Barbara F.
• Is Part Of: Fish & shellfish immunology. Issue 124(2022)
• Page Start: 28
• Page End: 38
• Publication Date: 2022-05
• Subjects: Spot lesion -- Chinook salmon -- Transcriptome -- Skin -- Mucin -- Annexin -- Cytokine; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2022.03.024
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Little is known about host responses of farmed Chinook salmon with skin lesions, despite the lesions being associated with increased water temperatures and elevated mortality rates. To address this shortfall, a transcriptomic approach was used to characterise the molecular landscape of spot lesions, the most commonly reported lesion type in New Zealand Chinook salmon, versus healthy appearing skin in fish with and without spot lesions. Many biological (gene ontology) pathways were enriched in lesion adjacent tissue, relative to control skin tissue, including proteolysis, fin regeneration, calcium ion binding, mitochondrial transport, actin cytoskeleton organisation, epithelium development, and tissue development. In terms of specific transcripts of interest, pro-inflammatory cytokines (interleukin 1β and tumour necrosis factor), annexin A1, mucin 2, and calreticulin were upregulated, while cathepsin H, mucin 5AC, and perforin 1 were downregulated in lesion tissue. In some instances, changes in gene expression were consistent between lesion and healthy appearing skin from the same fish relative to lesion free fish, suggesting that host responses weren't limited to the site of the lesion. Goblet cell density in skin histological sections was not different between skin sample types. Collectively, these results provide insights into the physiological changes associated with common spot lesions in farmed Chinook salmon. Highlights: Lesion enriched: proteolysis, mitochondrial transport, epithelium development. Pro-inflammatory cytokines were upregulated and mucins were downregulated adjacent to lesions. Gene expression was altered in healthy appearing skin in fish that had a lesion. Potential biomarkers have been identified to indicate lesion susceptibility.