Chronic exposure to tralopyril induced abnormal growth and calcium regulation of turbot (Scophthalmus maximus). (July 2022)

• Record Type: Journal Article
• Authors: Liu, Bin; Li, Ping; He, Shuwen; Xing, Shaoying; Chen, Chengzhuang; Liu, Ling; Li, Zhi-Hua
• Is Part Of: Chemosphere. Volume 299(2022)
• Publication Date: 2022-07
• Subjects: Tralopyril -- Marine fish -- RNA-Seq -- Physiological responses; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2022.134405
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Tralopyril is an emerging marine antifouling agent with limited data on its effects on fish growth and calcium regulation. To determine the changes induced by long-term exposure to tralopyril, multi-levels (such as molecular, biochemical, and individual levels) responses were measured in turbot at different concentrations (1 μg/L, 20 μg/L). The results showed that 1 μg/L mainly affected the immune response, while 20 μg/L affected the synthesis and metabolism of steroids and fat. However, different concentrations of tralopyril affected the synthesis, secretion and action of parathyroid hormone and growth hormone. The expression of GH/IGF axis gene and the level of growth hormone increased significantly, leading to abnormal growth. The energy tradeoff between immunity and growth at 1 μg/L tralopyril pressure may inhibit growth. The change of Ca 2+ level was accompanied by the disturbance of PTH-related gene expression. The results of molecular docking showed that the disturbance of Ca 2+ regulation might be attributed to the inhibition of vitamin D receptor by tralopyril, which affected the vitamin D signaling pathway. This study provides scientific data for the in-depth understanding and risk assessment of the toxicological effects of tralopyril and reveals the potential threat of tralopyril to environmental health. Graphical abstract: Image 1 Highlights: Tralopyril caused transcriptome changes in fish liver. Tralopyril induced GH elevation and GH/IGF axis gene disturbance, resulting in abnormal growth. Tralopyril interferes with vitamin D signaling pathway and PTH genes, causing Ca 2+ regulation disorder. Tralopyril is a potential threat to environmental health.