Biorefinery of apple pomace: New insights into xyloglucan building blocks. (15th August 2022)

• Record Type: Journal Article
• Authors: Chen, Mingrui; Mac-Béar, Jessica; Ropartz, David; Lahaye, Marc
• Is Part Of: Carbohydrate polymers. Volume 290(2022)
• Publication Date: 2022-08-15
• Subjects: Apple pomace biorefinery -- Hemicellulose sequential extraction -- Xyloglucan building motifs -- Unbranched glucan segments; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2022.119526
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: Within the apple pomace biorefinery cascade processing framework aiming at adding value to an agroindustrial waste, after pectin recovery, this study focused on hemicellulose. The structure of the major apple hemicellulose, xyloglucan (XyG), was assessed as a prerequisite to potential developments in industrial applications. DMSO-LiCl and 4 M KOH soluble hemicelluloses from pectin-extracted apple pomace were purified by anion exchange chromatography. XyG structure was assessed by coupling xyloglucanase and endo -β-1, 4-glucanase digestions to HPAEC and MALDI-TOF MS analyses. 71.9% of pomaces hemicellulose were recovered with starch. DMSO-LiCl and 4 M KOH soluble XyG exhibited Mw of 19 and 140 kDa, respectively. Besides the XXXG, XLXG, XXLG, XXFG, XLFG and XLLG structures, novel oligosaccharides with degree of polymerization of 6–10 were observed after xyloglucanase digestion. Cellobiose and cellotriose were revealed randomly distributed in XyG backbone and were more present in DMSO-LiCl soluble XyG. Residual pomace remains a potential source of other materials. Graphical abstract: Unlabelled Image