OsLTP47 may function in a lipid transfer relay essential for pollen wall development in rice. (May 2022)

• Record Type: Journal Article
• Authors: Chen, Libin; Ji, Chonghui; Zhou, Degui; Gou, Xin; Tang, Jianian; Jiang, Yongjie; Han, Jingluan; Liu, Yao-Guang; Chen, Letian; Xie, Yongyao
• Is Part Of: Journal of genetics and genomics. Volume 49:Number 5(2022)
• Page Start: 481
• Page End: 491
• Publication Date: 2022-05
• Subjects: Rice -- Pollen wall -- Lipid transfer protein -- Male sterility; Genetics -- Periodicals; Genomics -- Periodicals; 576.505
• DOI: 10.1016/j.jgg.2022.03.003
• Languages: English
• ISSNs: 1673-8527

Abstract

Abstract: In plants, lipid transfer proteins (LTPs) transport pollen wall constituents from the tapetum to the exine, a process essential for pollen wall development. However, the functional cooperation of different LTPs in pollen wall development is not well understood. In this study, we have identified and characterized a grass-specific LTP gene, OsLTP47, an important regulator of pollen wall formation in rice ( Oryza sativa ). OsLTP47 encodes a membrane-localized LTP and in vitro lipid-binding assays confirms that OsLTP47 has lipid-binding activity. Dysfunction of OsLTP47 causes disordered lipid metabolism and defective pollen walls, leading to male sterility. Yeast two-hybrid and pull-down assays reveal that OsLTP47 physically interacts with another LTP, OsC6. These findings suggest that the plasma membrane-localized OsLTP47 may function as a mediator in a lipid transfer relay through association with cytosolic and/or locular OsC6 for pollen wall development and that various LTPs may function in a coordinated manner to transport lipid molecules during pollen wall development.