Label-Free Ratiometric Electrochemiluminescent (ECL) Immunosensor for the Determination of Prostate Specific Antigen (PSA) in Serum. (24th May 2022)

• Record Type: Journal Article
• Authors: Cao, Jun-Tao; Liu, Xiang-Mei; Fu, Yi-Zhuo; Ren, Shu-Wei; Liu, Yan-Ming
• Is Part Of: Analytical letters. Volume 55:Number 11(2022)
• Page Start: 1810
• Page End: 1821
• Publication Date: 2022-05-24
• Subjects: cadmium sulfide quantum dot (CdS QD) modified glassy carbon electrode (GCE) -- electrochemiluminescent immunosensor -- human serum -- luminol–gold nanoparticles (AuNPs) -- prostate specific antigen (PSA); Chemistry, Analytic -- Periodicals; Chemistry, Analytic -- Abstracts; 543
• DOI: 10.1080/00032719.2022.2027957
• Languages: English
• ISSNs: 0003-2719

Abstract

Abstract: A spatial- and potential-resolved ratiometric electrochemiluminescent (ECL) immunosensor was developed for prostate specific antigen detection in a label-free mode. CdS quantum dots (CdS QDs) and luminol-gold nanoparticles (luminol-Au NPs) were the potential-resolved electrochemiluminescence emitters and H2 O2 was used as a co-reactant. The cathodic signal from the CdS QD electrode served as the working signal and the anodic signal from luminol-Au NPs as the reference. For prostate specific antigen detection, several concentrations of prostate specific antigen were immobilized on the CdS QDs modified electrode and a fixed concentration of the prostate specific antigen was modified on the luminol-Au NPs modified electrode. The prostate specific antigen levels were quantified by the electrochemiluminescence signal ratio of the cathodic and anodic (ECLcathodic /ECLanodic ). Under the optimized conditions, the ratio of the cathodic and anodic signals (ECLcathodic /ECLanodic ) had a linear relationship with the logarithm of (C, g mL −1 ) of prostate specific antigen concentration from 1.0 × 10 −11 to 1.0 × 10 −7 g mL −1 . The detection limit was 5.0 × 10 −12 g mL −1 . Prostate specific antigen was determined in human serum and the recoveries were from 80.0% to 107.0%. The label-free electrochemiluminescence immunosensor offers simplicity and reliability for human serum with potential for clinical application.