Molecular delineation, expression profiling, immune response, and anti-apoptotic function of a novel clusterin homolog from big-belly seahorse (Hippocampus abdominalis). Issue 124 (May 2022)

• Record Type: Journal Article
• Authors: Wijerathna, H.M.S.M.; Nadarajapillai, Kishanthini; Udayantha, H.M.V.; Kasthuriarachchi, T.D.W.; Shanaka, K.A.S.N.; Kwon, Hyukjae; Wan, Qiang; Lee, Jehee
• Is Part Of: Fish & shellfish immunology. Issue 124(2022)
• Page Start: 289
• Page End: 299
• Publication Date: 2022-05
• Subjects: Hippocampus abdominalis -- Clusterin -- Oxidative stress -- Anti-apoptotic effect -- Innate immune responses; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2022.04.015
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Clusterin (CLU) is a glycoprotein that contains α- and β-chains. CLU exerts multifunctional activities and plays a role in different cell signaling pathways that are associated with various diseases such as proteotoxic and oxidative stress, as well as cell death and survival. However, its role in marine teleost fish remains unclear. Therefore, the present study was carried out to characterize and investigate the immune responses and anti-apoptotic effects of CLU of the big-belly seahorse ( Hippocampus abdominalis ) (HaCLU) on oxidative stress-induced cell death. The HaCLU open reading frame was 1389 bp long and encoded a protein with 462 amino acids, a molecular weight of 51.28 kDa and an isoelectric point of 5.41. In-silico results demonstrated that HaCLU has a signal peptide in the 1–29 amino acid region, while the α- and β-chains were in the 34–227 and 228–455 amino acid regions, respectively. Multiple sequence alignment clarified the low homology of the α-chain with other orthologs. The highest HaCLU mRNA expression level was observed in the liver, followed by the heart, spleen, and brain tissues of healthy big-belly seahorses. Further, HaCLU mRNA expression level was elevated in the liver in response to different stimuli, including lipopolysaccharides, polyinosinic:polycytidylic acid, Edwardsiella tarda, and Streptococcus iniae . HaCLU potentiates cell viability and weakens chromatin condensation in the nucleus of FHM cells following H2 O2 -induced oxidative stress and subsequent cell death. HaCLU overexpression resulted in a reduced Bax/Bcl- 2 mRNA expression ratio. This study revealed the role of HaCLU in immune regulation against pathogenic infections and its anti-apoptotic effects on oxidative stress-induced cell death. Highlights: Clusterin was identified in Hippocampus abdominalis and characterized. Clusterin shows highest expression in liver tissue of healthy seahorse followed by heart, spleen, and brain. Immune stimulants significantly modulate the Clusterin in liver tissue. Overexpressed Clusterin exhibit anti-apoptotic effect on oxidative stress-induced cell death.