A conformational study of the 10–23 DNAzyme via programmed DNA self-assembly. Issue 42 (6th May 2022)

• Record Type: Journal Article
• Authors: Mao, Dake; Li, Qian; Li, Qian; Wang, Pengfei; Mao, Chengde
• Is Part Of: Chemical communications. Volume 58:Issue 42(2022)
• Page Start: 6188
• Page End: 6191
• Publication Date: 2022-05-06
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d2cc01144a
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : We have developed a strategy to study the interhelical angle of two DNA duplexes in nucleic acids as exemplified by the 10–23 DNAzyme. The nucleic acids are programmed to assemble into large 2D arrays to reduce random conformation fluctuation and facilitate the conformation study. Abstract : This communication measures the inter-helical angle of the 10–23 DNAzyme–substrate complex by atomic force microscopy (AFM). Herein, we have devised a strategy to assemble the DNAzyme–substrate complex into a periodic DNA 2D array, which allows reliable study of the conformation of the 10–23 DNAzyme by AFM imaging and fast Fourier transform (FFT). Specifically, the angle between the two flanking helical domains of the catalytic core has been determined via the repeating distance of the 2D array. We expect that the same strategy can generally be applicable for studying other nucleic acid structures.