Design and fabrication of chitosan-based AIE active micelles for bioimaging and intelligent delivery of paclitaxel. (15th August 2022)

• Record Type: Journal Article
• Authors: Xu, Yang; Liang, Na; Liu, Jiyang; Gong, Xianfeng; Yan, Pengfei; Sun, Shaoping
• Is Part Of: Carbohydrate polymers. Volume 290(2022)
• Publication Date: 2022-08-15
• Subjects: AIE aggregation-induced emission -- BT biotin -- CB cetyl 4-formylbenzoate -- CLSM confocal laser scanning microscopy -- CMC critical micelle concentration -- CS chitosan -- CS-BT biotinylated chitosan -- CS-BT-HBS 4-(2-hydroxyethoxy) benzophenonesalicylaldazide modified biotinylated chitosan -- CS-BT-HBS-CB cetyl 4-formylbenzoate alkyl and 4-(2-hydroxyethoxy) benzophenonesalicylaldazide modified biotinylated chitosan -- DCM dichloromethane -- DL drug loading -- DLS dynamic light scattering -- DMAP 4-dimethylaminopyridine -- DMEM Dulbecco's modified Eagle's medium -- DPAS diphenylketone azine salicylaldehyde -- EDC 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride -- EE encapsulation efficiency -- EPR enhanced permeability and retention -- ESIPT excited-state intramolecular proton transfer -- FBS fetal bovine serum -- FL fluorescence -- GSIPT ground state intramolecular proton transfer -- HB 4-(2-hydroxyethoxy) benzophenone -- HBH 4-(2-hydroxyethoxy) benzophenone hydrazone -- HBS 4-(2-hydroxyethoxy) benzophenonesalicylaldazide -- NHS N-hydroxysuccinimide -- PBS phosphate buffered saline -- PTX paclitaxel -- RIM restriction of intramolecular motions -- RPMI Roswell Park Memorial Institute -- SD substitution degree -- TEM transmission electron microscope -- THF tetrahydrofuran -- TIR tumor inhibition rate; Polymeric micelles -- Aggregation-induced emission (AIE) -- Bioimaging -- Tumor targeting -- pH responsive; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2022.119509
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: In this study, cetyl 4-formylbenzoate alkyl and 4-(2-hydroxyethoxy) benzophenonesalicylaldazide modified biotinylated chitosan (CS-BT-HBS-CB) featured with aggregation-induced emission (AIE) characteristic, active tumor-targeting ability and pH-responsive drug release property was designed and synthesized. The polymer was fabricated by introducing hydrophobic segment, tumor targeting ligand, acid-sensitive bond and AIE fluorophore to the backbone of chitosan. Due to its amphiphilicity, the polymer could self-assemble into micelles and encapsulate paclitaxel (PTX) to form PTX-loaded CS-BT-HBS-CB micelles. The mean size of the micelles was 167 nm, which was beneficial to the EPR effect. Moreover, with the help of above functional groups, the micelles exhibited excellent AIE effect, triggered drug release behavior by acidic condition, selective internalization by MCF-7 cells and excellent cellular imaging capability. In vivo studies revealed that the PTX-loaded CS-BT-HBS-CB micelles could enhance the antitumor efficacy with low systemic toxicity. This micellar system would be a potential candidate for cancer therapy and bioimaging. Graphical abstract: Unlabelled Image