Single-channel digital LAMP multiplexing using amplification curve analysis. Issue 3 (22nd April 2022)

• Record Type: Journal Article
• Authors: Malpartida-Cardenas, Kenny; Miglietta, Luca; Peng, Tianyi; Moniri, Ahmad; Holmes, Alison; Georgiou, Pantelis; Rodriguez-Manzano, Jesus
• Is Part Of: Sensors & diagnostics. Volume 1:Issue 3(2022)
• Page Start: 465
• Page End: 468
• Publication Date: 2022-04-22
• Subjects: Biosensors; Periodicals; Diagnosis; Chemical detectors; 616.0756
• DOI: 10.1039/d2sd00038e
• Languages: English
• ISSNs: 2635-0998

Abstract

Abstract : We demonstrate LAMP multiplexing (5-plex) in a single reaction with a single fluorescent channel using the machine learning-based method amplification curve analysis, showing a classification accuracy of 91.33% for detection of respiratory pathogens. Abstract : Loop-mediated isothermal amplification assays are currently limited to one target per reaction in the absence of melting curve analysis, molecular probes or restriction enzyme digestion. Here, we demonstrate multiplexing of five targets in a single fluorescent channel using digital LAMP and the machine learning-based method amplification curve analysis, resulting in a classification accuracy of 91.33% on 54 186 positive amplification events.