Sialyl-Tn antigen facilitates extracellular vesicle-mediated transfer of FAK and enhances motility of recipient cells. (1st February 2022)

• Record Type: Journal Article
• Authors: Nagao, Keisuke; Maeda, Kento; Hosomi, Kasumi; Morioka, Kaito; Inuzuka, Tatsutoshi; Ohtsubo, Kazuaki
• Is Part Of: Journal of biochemistry. Volume 171:Number 5(2022)
• Page Start: 543
• Page End: 554
• Publication Date: 2022-02-01
• Subjects: cellular motility -- focal adhesion kinase -- TSAP6 -- extracellular vesicle -- Sialyl-Tn antigen; Biochemistry -- Periodicals; Biochemistry -- Periodicals; Electronic journals; 572.05
• DOI: 10.1093/jb/mvac008
• Languages: English
• ISSNs: 0021-924X

Abstract

Abstract: Protein glycosylation plays a pivotal role in tumour development by modulating molecular interactions and cellular signals. Sialyl-Tn (sTn) antigen is a tumour-associating carbohydrate epitope whose expression correlates with metastasis and poor prognosis of various cancers; however, its pathophysiological function is poorly understood. Extracellular vesicles (EVs) derived from cancer cells act as a signal mediator amongst tumour microenvironments by transferring cargo molecules. sTn antigen has been found in the glycans of EVs, thereby the functional relevance of sTn antigen to the regulation of tumour microenvironments could be expected. In the present study, we showed that sTn antigen induced TP53 and tumour suppressor–activated pathway 6 (TSAP6) and consequently enhanced EV production. Besides, the genetic attenuation of TSAP6 resulted in the reduction of the EV production in the sTn antigen expressing cells. The enhanced EV production in the sTn antigen–expressing cells consequently augmented the delivery of EVs to recipient cells. The produced EVs selectively and abundantly encased focal adhesion kinase and transferred it to EV-recipient cells, and thus, their cellular motility was enhanced. These findings would contribute to facilitate the elucidation of the pathophysiological significance of the sTn antigen in the tumour microenvironments and tumour development. Graphical Abstract: