A sensitive "on-off-on" fluorescent probe for sequential Cu2+/S2− detection in actual water samples and living cells. (4th June 2022)

• Record Type: Journal Article
• Authors: Qiao, Yi-Jie; Xu, Fang-Zhou; Chen, Yun; Wang, Zhi-Qiang; Gong, Xue-Qing; Wang, Cheng-Yun
• Is Part Of: Tetrahedron. Volume 115(2022)
• Publication Date: 2022-06-04
• Subjects: Fluorescent probe -- Copper ion (II) -- Sulfide ion (II) -- Sequential detection; Chemistry, Organic -- Periodicals; 547.005
• DOI: 10.1016/j.tet.2022.132791
• Languages: English
• ISSNs: 0040-4020

Abstract

Abstract: Aquatic organism is far less tolerant to Cu 2+ than that of mammals, and the excessive Cu 2+ can also be harmful to human by bioconcentration. Herein, a fluorescent probe (DPA-SAA ) that can be easily synthesized was developed for detecting traces of Cu 2+ in actual aqueous environment and cells. The experimental and theoretical results showed that the fluorescence of DPA-SAA could be evidently quenched by only 1% equivalent of Cu 2+ . The limit of detection (LOD) was calculated to be 0.66 nM, which is far below the Chinese standard of fishery water quality (157 nM). Simultaneously, the Cu 2+ of complex DPA-SAA /Cu 2+ could be captured by S 2−, thus realizing sequential S 2− detection with a low LOD of 13.93 nM. Moreover, DPA-SAA was proved qualified for Cu 2+ detection in actual water samples with good recovery, and sequential Cu 2+ /S 2− sensing in living cells. Graphical abstract: Image 1 Highlights: A sequential fluorescent probe applied in actual water samples and living cells. Extremely low detection limit for Cu 2+ (0.66 nM) and S 2− (13.93 nM). Easy preparation. High sensitivity and selectivity.