Effectiveness of layered inoculation in solid-state anaerobic co-digestion of pig manure and corn straw: Focus on macro-, micro-, and genetic-levels. (July 2022)

• Record Type: Journal Article
• Authors: Wang, Siqi; Kong, Dewang; Zhang, Keqiang; Chang, Xingping; Lu, Zhenwei; Du, Lianzhu
• Is Part Of: Bioresource technology. Volume 355(2022)
• Publication Date: 2022-07
• Subjects: Layered inoculation -- Solid-state anaerobic co-digestion -- Methanation performance -- Microbial dynamics -- Methanogenic pathways; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2022.127262
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Layered inoculation improved volatile fatty acid utilization in anaerobic digestion. Adverse effects of high total ammonium nitrogen concentrations was prevented. Microbes from inocula efficiently inoculated into substrates with mass exchange. Hydrolysis, acidification, and methanogenesis were synergistic from day 0 to 13. Acetoclastic pathway was favored in the substrates from day 0 to 13. Abstract: Layered inoculation can achieve rapid start-up and promote methanation performance of anaerobic digesters. Daily specific methane yield (SMY) rapidly increased to 2.93 mL/g VS/d during 0–13 days, and cumulative SMY reached 212 mL/g VS in the solid-state anaerobic co-digestion (SS-AcoD) of pig manure and corn straw. Data were collected at macro-, micro-, and genetic-levels of each substrate layer. The results showed that layered inoculation could improve volatile fatty acids utilization and prevent adverse effects of high total ammonium nitrogen concentrations. Layered inoculation accelerated hydrolysis, acidification, and methanogenesis of substrates, as evidenced by the efficient inoculation of Bacteroidetes, Anaerolineales, Methanosphaerula, and Methanothrix, which were primarily from inocula. The various stages of SS-AcoD were synergistically initiated during the first 13 days, and acetoclastic pathway was boosted. These results further explain why layered inoculation is an efficient method for improving methanation performance of SS-AcoD and achieving efficient utilization of organic solid waste.