Dermal fibroblast from superficial layers of pig skin exhibits more proliferative capacity than that from deep layers. Issue 2 (May 2022)

• Record Type: Journal Article
• Authors: Zuo, Yanhai; Yi, Lei; Lu, Shuliang
• Is Part Of: Journal of tissue viability. Volume 31:Issue 2(2022)
• Page Start: 278
• Page End: 285
• Publication Date: 2022-05
• Subjects: Animal model -- Dermal fibroblast -- Heterogeneity -- Pig -- Proliferating cell nuclear antigen -- Skin; DF dermal fibroblast -- PCNA proliferating cell nuclear antigen -- PBS phosphate buffer saline solution -- FRDP female red Duroc pig -- HE hematoxylin and eosin; Wounds and injuries -- Periodicals; Ulcers -- Periodicals; Bedsores -- Periodicals; Bedsores; Ulcers; Wounds and injuries; Electronic journals; Periodicals; 617.1406
• DOI: 10.1016/j.jtv.2021.12.001
• Languages: English
• ISSNs: 0965-206X

Abstract

Abstract: Objective: To further examine the feasibility of using pigs as an animal model for the study of dermal fibroblast heterogeneity and to explore the proliferative capacity of dermal fibroblasts from different layers of pig skin in vitro and in vivo. Material and methods: Cultured superficial and deep dermal fibroblasts were subjected to cell growth assay, cell cycle analysis, immunocytochemical staining and western blotting for proliferating cell nuclear antigens. Moreover, skin samples autografted with superficial/deep dermal fibroblasts were subjected to immunohistochemical staining and western blotting for proliferating cell nuclear antigen. Results: The cell growth assay showed that the growth curve of the superficial dermal fibroblast was progressively higher than that of the deep layer. The cell cycle analysis showed that the (G2+S) percentage of the superficial dermal fibroblasts was significantly higher than that of the deep layer fibroblasts. The immunocytochemical staining and western blotting showed that the expression of proliferating cell nuclear antigen in the cultured superficial dermal fibroblast was significantly higher than that of the deep layer cells. The immunohistochemical staining showed that the positive rate of proliferating cell nuclear antigen in the skin samples autografted with the superficial dermal fibroblast was significantly higher than that of the deep layer. Conclusions: This study has demonstrated that similar to human dermal fibroblasts, dermal fibroblasts from different layers of pig skin exhibit distinct proliferative capacity, which increases the feasibility of using pigs as an animal model for future studies on the heterogeneity of dermal fibroblasts. Highlights: Dermal fibroblasts from different layers of pig skin exhibit distinct proliferative capacity. This study increases the feasibility of using pigs as an animal model for dermal fibroblasts heterogeneity. This study might shed light on the selection of more suitable dermal fibroblasts according to the therapeutic indication.