Prospective study of serum and aqueous humour anti‐Hsp70.1 IgG antibody levels in ocular toxoplasmosis. (7th August 2020)

• Record Type: Journal Article
• Authors: Chiquet, Christophe; Chumpitazi, Bernabé; Vilgrain, Isabelle; Lesoin, Anne; Fricker‐Hidalgo, Helene; Brenier‐Pinchart, Marie‐Pierre; Vasseneix, Caroline; Savy, Olivier; Campolmi, Nelly; Gain, Philippe; Flori, Pierre; Aptel, Florent; Pelloux, Hervé; Bouillet, Laurence
• Is Part Of: Parasite immunology. Volume 42:Number 10(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-08-07
• Subjects: anti‐Hsp70.1 antibody -- diagnosis -- ocular toxoplasmosis -- Toxoplasma gondii -- uveitis; Veterinary parasitology -- Immunological aspects -- Periodicals; Host-parasite relationships -- Immunological aspects -- Periodicals; 571.96
• DOI: 10.1111/pim.12771
• Languages: English
• ISSNs: 0141-9838

Abstract

Abstract: Aims: We evaluate whether the serum and aqueous humour (AH) level of IgG anti‐Hsp70.1 antibodies improved the biological diagnosis of ocular toxoplasmosis. Methods and results: In this prospective cross‐sectional and multicentre study, serum and AH were collected at the time of active uveitis. Anti‐Hsp70.1‐antibody levels were determined by ELISA. Patients with confirmed (Group A1, n = 21) or suspected ocular toxoplasmosis (group A2, n = 30) were enrolled, as well as a control group of patients with cataract (group B, n = 42). Serum IgG anti‐Hsp70.1 antibody levels were not significantly different within the group of uveitis patients (A1, n = 21 vs A2, n = 30, P = .8) and were significantly associated with the affected retinal zone ( P = .006) and with the size of the retinal lesion ( P = .03). Serum anti‐Hsp70.1 antibody level was positive in 10 out of the 18 patients of group A2. Significant anti‐Hsp‐70.1 antibody level in AH was reported in only three patients (3 eyes) with confirmed ocular toxoplasmosis. Conclusion: While the level of IgG anti‐Hsp‐70.1 antibody in AH did not improve the laboratory diagnosis of ocular toxoplasmosis, its level in serum was of major significance for retinal damage diagnosis.