Biochemical characterization of an engineered bifunctional xylanase/feruloyl esterase and its synergistic effects with cellulase on lignocellulose hydrolysis. (July 2022)

• Record Type: Journal Article
• Authors: Wang, Hongling; Qi, Xianghui; Gao, Song; Zhang, Yifeng; An, Yingfeng
• Is Part Of: Bioresource technology. Volume 355(2022)
• Publication Date: 2022-07
• Subjects: Agricultural residues -- Bifunctional xylanase/feruloyl esterase -- Domain truncation -- Lignocellulose hydrolysis; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2022.127244
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: A novel and more efficient bifunctional xylanase/feruloyl esterase XynII-Fae was constructed. Synergistic action of XynII-Fae with cellulase could dramatically improve the hydrolysis efficiency of lignocellulose. Replacement with XynII-Fae increased glucan and xylan conversion by 54.3% and 125.1%. XynII-Fae enhanced cellulase hydrolysis against different agricultural residues. Abstract: Herein, the xylanase and feruloyl esterase domains of the xylanase/feruloyl esterase bifunctional enzyme (Xyn-Fae) from Prevotella ruminicola 23 were identified using N- and C-terminal truncation mutagenesis. In addition, a novel and more efficient xylanase/feruloyl esterase bifunctional enzyme XynII-Fae was constructed, and its synergistic action with a commercial cellulase for lignocellulose hydrolysis was studied. When 40% cellulase was replaced by XynII-Fae, the production of reducing sugars increased by 65% than that with the cellulase alone, and the conversions of xylan and glucan were increased by 125.1% and 54.3%, respectively. When 80% cellulase was substituted by XynII-Fae, up to 43.5 μg/mL ferulic acid and 418.7 μg/mL acetic acid were obtained. The XynII-Fae could also accelerate the hydrolysis of wheat straw and sugarcane bagasse with commercial cellulase. These results indicated that the synergistic action of XynII-Fae with cellulase could dramatically improve the hydrolysis efficiency of lignocellulose, showing the great potential for industrial applications.