Plastid phylogeography of Delphinium fissum subsp. sordidum and the series Fissa (Ranunculaceae) in the Iberian Peninsula: implications for conservation. Issue 3 (3rd July 2019)

• Record Type: Journal Article
• Authors: Ramírez-Rodríguez, Rubén; Jiménez, Juan Francisco; Amich, Francisco; Sánchez-Gómez, Pedro
• Is Part Of: Botany letters. Volume 166:Issue 3(2019)
• Page Start: 345
• Page End: 355
• Publication Date: 2019-07-03
• Subjects: Delphinium series Fissa -- genetic diversity -- cpDNA -- haplotype network -- divergence time estimation -- conservation genetics -- biodiversity conservation; Botany -- Periodicals; Botany; Botany; Electronic journals; Periodicals; Periodical; 580
• DOI: 10.1080/23818107.2019.1663447
• Languages: English
• ISSNs: 2381-8107

Abstract

ABSTRACT: We study the phylogeography of the series Fissa species in the Iberian Peninsula. This group constitutes a complex formed by three Iberian endemic and threatened taxa with restricted distribution ranges and relatively small population sizes. We amplify and sequence two plastid regions, trn S- trn G and psb A- trn H from 15 sampling locations (n = 45). A total of 15 haplotypes are detected. The median-joining phylogenetic network shows two main haplotype lineages: one western corresponding to the western taxon, D. fissum subsp. sordidum, and the other eastern corresponding to eastern taxa D. bolosii and D. mansanetianum . AMOVA analysis with 94.32% of the total variation and SAMOVA analysis with a Fct value of 0.943 support the presence of these well-defined lineages. We report high values of total gene diversity (hT = 0.959). Haplotype diversity within populations is low (hS = 0.311). On the contrary, differentiation among populations (GST = 0.675) and Fixation Index (FST = 0.989) are high. These values mean that gene flow among populations is limited. Estimation of divergence times shows that the split between D. fissum subsp. sordidum and the two other taxa ( D. bolosii and D. mansanetianum ) took place during the transition between Pliocene and Pleistocene (approximately 2.67 Ma). Negative Tajima's D-values test (Tajima's D = -0.108, P ≥ 0.1) and unimodal mismatch distribution suggest expansion range for D. fissum subsp. sordidum . We advocate to draw up recovery plans for series Fissa species in the Iberian Peninsula, especially for D. fissum subsp. sordidum .