Oxytocin modulation in the medial prefrontal cortex of pair-exposed rats during fear conditioning. (July 2022)

• Record Type: Journal Article
• Authors: Jang, Minji; Jung, Taesub; Jeong, Yujeong; Byun, Younsoo; Noh, Jihyun
• Is Part Of: Psychoneuroendocrinology. Volume 141(2022)
• Publication Date: 2022-07
• Subjects: Cortex -- Oxytocin -- Passive avoidance -- Social buffering -- Social interaction -- Social tie; Psychoneuroendocrinology -- Periodicals; Endocrinology -- Periodicals; Neurology -- Periodicals; Psychiatry -- Periodicals; Neuropsychoendocrinologie -- Périodiques; 616.8
• DOI: 10.1016/j.psyneuen.2022.105752
• Languages: English
• ISSNs: 0306-4530

Abstract

Abstract: Introduction: Social buffering is the phenomenon, in which stress and fear reactions caused by exposure to stressful stimuli when animals are exposed to homogeneous relationships are attenuated. Social buffering reduces fear memory behavior such as escape, avoidance, and freezing behavior in rodents due to social existence. Here, we aimed to determine alterations of fear behavior and neural activity in the medial prefrontal cortex (mPFC) in response to the presence of another rat in fear-exposed conditions and to confirm the role of oxytocin in mPFC in regulating social buffering. Methods: We performed a passive avoidance test and determined positive c-Fos expression in single- and pair-exposed rats. Anisomycin (a protein synthesis inhibitor) and oxytocin receptor regulators (carbetocin; agonist and atosiban; antagonist) were microinjected into the mPFC to clarify the role of oxytocin in the mPFC. Results: While single-exposed rats showed a significant increase in both freezing and passive avoidance behaviors compared to control rats, pair-exposed rats showed significantly less fear behavior compared to single-exposed rats. The c-Fos expression in the prelimbic (PL) mPFC was significantly increased in pair-exposed rats compared to that in control and single-exposed rats. The pair-exposed effect was blocked by anisomycin injections into the PL mPFC of pair-exposed rats. Furthermore, when a carbetocin was injected into the PL mPFC in single-exposed rats, fear behavior was decreased, and these changes were blocked by atosiban. Discussion: Our findings suggest that reduction of fear-related behavior induced by acute pair-exposure is mediated by oxytocin receptors in the PL mPFC. Pair exposure with conspecifics during fear-inducing situations helps coping with fear by significantly increasing the role of oxytocin in the PL mPFC. Highlights: Socially paired rats show less fear during fear-conditioning than single ones. In paired rats, medial prefrontal cortex (mPFC) c-Fos expression is increased. Fear reduction in paired rats is blocked by a protein synthesis inhibitor. Fear reduction is induced by mPFC infusion of the oxytocin receptor agonist carbetocin. Fear reduction in paired rats is blocked by the oxytocin receptor antagonist atosiban.